Plexin B2 Antibody (3E7) - BSA Free
Novus Biologicals | Catalog # NBP1-43310
![Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]](https://resources.rndsystems.com/images/products/Plexin-B2-Antibody-3E7-Flow-Cytometry-NBP1-43310-img0004.jpg "Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]")
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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry, Immunoprecipitation
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Armenian Hamster IgG Clone # 3E7
Format
BSA Free
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Product Specifications
Immunogen
The immunogen for this antibody was PlexinB2.
Clonality
Monoclonal
Host
Armenian Hamster
Isotype
IgG
Scientific Data Images for Plexin B2 Antibody (3E7) - BSA Free
Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]
Flow Cytometry: Plexin B2 Antibody (3E7) [NBP1-43310] - Analysis using the Biotin conjugate of NBP1-43310. Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Armenian Hamster IgG Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse Plexin-B2 Biotin (right) followed by Streptavidin PE. Total viable cells were used for analysis.![Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]](https://resources.rndsystems.com/images/products/Plexin-B2-Antibody-3E7-Flow-Cytometry-NBP1-43310-img0002.jpg "Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]")
Flow Cytometry: Plexin B2 Antibody (3E7) - BSA Free [NBP1-43310]
Flow Cytometry: Plexin B2 Antibody (3E7) [NBP1-43310] - Staining of the PDV cell line with 0.25 ug of Armenian Hamster IgG Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse Plexin-B2 Purified (filled histogram) followed by Anti-Armenian Hamster IgG Biotin and Streptavidin PE. Total viable cells were used for analysis.Applications for Plexin B2 Antibody (3E7) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:10-1:1000
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS (pH 7.2)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Plexin B2
Alternate Names
MM1, PLEXB2, PLXNB2
Gene Symbol
PLXNB2
Additional Plexin B2 Products
Product Documents for Plexin B2 Antibody (3E7) - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Plexin B2 Antibody (3E7) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Plexin B2 Antibody (3E7) - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Liperfluo
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Plexin B2 Antibody (3E7) - BSA Free
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Q: I was wondering if you had an antibody that would recognize a fusion protein Plexin-B2? Our protein is from mouse Plexin-B2 (NP_001152993) amino acid Leu20-Trp1029. Do any of your Plexin-B2 antibodies recognize this amino acid stretch? I was hoping to use this antibody for IP.
A: NBP1-43310 uses the extracellular domain of Plexin B2 as the immunogen. This region is amino acids 20-1197. It is not epitope mapped but this is the only product we sell that is within your amino acid range. Chances are it maps to your region but there is a slight chance it might map to amino acids 1029-1197.
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