PLK1 [p Thr210] Antibody - Azide Free

Novus Biologicals | Catalog # NB100-61108

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Primate (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH. Accession # P53350

Modification

p Thr210

Marker

Mitosis Marker

Specificity

Specific for endogenous levels of the ~66 kDa PLK1 phosphorylated at Thr210. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK1 [p Thr210] Antibody - Azide Free

Western Blot: PLK1 [p Thr210] Antibody [NB100-61108]

Western Blot: PLK1 [p Thr210] Antibody [NB100-61108]

Western Blot: PLK1 [p Thr210] Antibody [NB100-61108] - Rat synaptic membrane showing specific immunolabeling of the ~66 k PLK protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Thr210 PLK antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Applications for PLK1 [p Thr210] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLK1

Polo-like kinases are important regulators of cell cycle progression. PLK1 is a highly conserved Ser/Thr kinase that has essential roles in the formation of mitotic bipolar spindles (van Vugt et al., 2004). Deregulated expression of PLK's is detected in many types of cancer and associated with oncogenesis (Takei et al., 2005). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (van de Weerdt et al., 2005).

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Entrez Gene IDs

5347 (Human); 25515 (Rat)

Gene Symbol

PLK1

UniProt

P53350

Additional PLK1 Products

Product Documents for PLK1 [p Thr210] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for PLK1 [p Thr210] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

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