PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-278

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Rat, Xenopus

Predicted:

Human (100%), Mouse (100%), Rat (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all PLK1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH. Accession # P70032

Modification

p Ser482, p Ser486, p Ser490

Localization

Nuclear

Marker

Mitosis Marker

Specificity

Specific for endogenous levels of the ~120k PLK1 protein phosphorylated at Ser482,486,490.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278]

Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278]

Western Blot: PLK1 [p Ser482, p Ser486, p Ser490] Antibody [NB300-278] - Analysis of rat hippocampal membrane lysate showing specific immunolabeling of the ~120 kDa Polo-Like Kinase Kinase phosphorylated at Ser482,486,490 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.

Applications for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PLK1

Considerable evidence indicates that a Polo-Like Kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK.

Long Name

Polo-like Kinase 1

Alternate Names

STPK13

Gene Symbol

PLK1

UniProt

P53350, P70032 (Xenopus)

Additional PLK1 Products

Product Documents for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Intracellular Kinases

Citations for PLK1 [p Ser482, p Ser486, p Ser490] Antibody - Azide Free

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