PLK4 Antibody (1C8) - Azide and BSA Free
Novus Biologicals | Catalog # H00010733-M01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1C8
Format
Azide and BSA Free
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Product Specifications
Immunogen
PLK4 (AAH36023, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHPSILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSE
Reactivity Notes
Human. Other species not tested.
Specificity
PLK4 (1C8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for PLK4 Antibody (1C8) - Azide and BSA Free
Western Blot: PLK4 Antibody (1C8) [H00010733-M01]
Western Blot: PLK4 Antibody (1C8) [H00010733-M01] - Western Blot analysis of PLK4 expression in Hela NE ( Cat # L013V3 ).
Sandwich ELISA: PLK4 Antibody (1C8) [H00010733-M01] - Detection limit for recombinant GST tagged PLK4 is approximately 0.03ng/ml as a capture antibody.
Applications for PLK4 Antibody (1C8) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactive against cell lysate for western blot. It has also been used for ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: PLK4
Long Name
Serine/threonine-protein kinase PLK4
Alternate Names
PLK-4, Polo-like kinase 4, SAK, STK18
Entrez Gene IDs
10733 (Human)
Gene Symbol
PLK4
OMIM
605031 (Human)
UniProt
Additional PLK4 Products
Product Documents for PLK4 Antibody (1C8) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PLK4 Antibody (1C8) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for PLK4 Antibody (1C8) - Azide and BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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