PMCA1 Antibody

Novus Biologicals | Catalog # NBP1-97754

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PMCA1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-KQDGAIENRNKAKAQD corresponding to internal region according to NP_001673.2.

Specificity

This antibody is expected to recognize both reported isoforms (NP_001001323.1; NP_001673.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for PMCA1 Antibody

Western Blot: PMCA1 Antibody [NBP1-97754]

Western Blot: PMCA1 Antibody [NBP1-97754]

Western Blot: PMCA1 Antibody [NBP1-97754] - (0.03ug/ml) Analysis of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for PMCA1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.03 - 0.1 ug/ml
Application Notes
WB: Approx. 150 kDa band observed in HeLa lysates (calculated MW of 135 kDa band according to NP_001673.2).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PMCA1

The calcium pump of the plasma membrane (PMCA ATPase) pumps calcium from the cytosol to the extracellular space. This membrane-bound enzyme is related to a number of other ATPases including the sarcoplasmic and endoplasmic reticulum calcium (SERCA) ATPase and the sodium/potassium pump. Studies indicate that there are four known isoforms of the PMCA ATPase which are encoded by a multigene family.

Alternate Names

ATPase, Ca++ transporting, plasma membrane 1, EC 3.6.3, EC 3.6.3.8, plasma membrane calcium ATPase, Plasma membrane calcium ATPase isoform 1, plasma membrane calcium pump, Plasma membrane calcium pump isoform 1, plasma membrane calcium-ATPase, PMCA1kb, PMCA1plasma membrane calcium-transporting ATPase 1

Entrez Gene IDs

490 (Human); 67972 (Mouse); 29598 (Rat)

Gene Symbol

ATP2B1

UniProt

NP_001001323.1 (Human)

Additional PMCA1 Products

Product Documents for PMCA1 Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for PMCA1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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