PNK Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-12588

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PNK Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with E. coli-derived Human PNK fragment

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Protein A & Antigen Affinity purified

Scientific Data Images for PNK Antibody - BSA Free

Western Blot: PNK Antibody [NBP3-12588]

Western Blot: PNK Antibody [NBP3-12588]

Western Blot: PNK Antibody [NBP3-12588] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: PC-3 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:57 kDaObserved band size:60 kDa(We are unsure as to the identity of these extra bands.)
Immunoprecipitation: PNK Antibody [NBP3-12588]

Immunoprecipitation: PNK Antibody [NBP3-12588]

Immunoprecipitation: PNK Antibody [NBP3-12588] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-PNKP rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-PNKP rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 57 kDaObserved band size :60 kDa

Applications for PNK Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5uL/mg of uLysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PNK

PNK catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). Reduces laminin matrix deposition and cell adhesion to laminin, but not to fi

Long Name

Bifunctional polynucleotide phosphatase/kinase

Alternate Names

PNKP

Gene Symbol

PNKP

Additional PNK Products

Product Documents for PNK Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PNK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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