Podocalyxin Like Antibody (PODXL/2185)

Novus Biologicals | Catalog # NBP2-79749

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Protein Array

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # PODXL/2185
View all Podocalyxin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human Podocalyxin Like (around aa 310-447) (exact sequence is proprietary) (Uniprot: O00592)

Localization

Cell Surface, Cytoplasm

Marker

Hematopoietic Stem Cell Marker

Specificity

Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression of podocalyxin may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79884)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Podocalyxin Like Antibody (PODXL/2185)

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Formalin-fixed, paraffin-embedded human Endometrium stained with Podocalyxin Like Antibody (PODXL/2185).
Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Immunohistochemistry-Paraffin: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Formalin-fixed, paraffin-embedded human Placenta stained with Podocalyxin Like Antibody (PODXL/2185).
Protein Array: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Protein Array: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749]

Protein Array: Podocalyxin Like Antibody (PODXL/2185) [NBP2-79749] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Podocalyxin Like Antibody (PODXL/2185). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Podocalyxin Like Antibody (PODXL/2185)

Application
Recommended Usage

Immunohistochemistry

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Podocalyxin

Podocalyxin (PODXL) is a CD34 family member expressed by podocytes, vascular endothelium, mesothelium, and a subset of hematopoietic progenitors. It is the major sialoprotein in the glycocalyx of glomerular podocytes. The physiological role of podocalyxin in leukemic blasts is unknown. However, it is thought of as a candidate prostate cancer tumor aggressiveness gene. Podocalyxin appears to complement CD34 as a useful cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells.

Alternate Names

GCTM, PCLP, POD XL, PODXL

Gene Symbol

PODXL

UniProt

O00592 (Human)

Additional Podocalyxin Products

Product Documents for Podocalyxin Like Antibody (PODXL/2185)

Certificate of Analysis

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Product Specific Notices for Podocalyxin Like Antibody (PODXL/2185)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Podocalyxin Like Antibody (PODXL/2185)

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Protocols

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