Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Primate (95%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot

Cited:

Western Blot, Chemotaxis

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all POT1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human POT1 protein sequence (between residues 200-300). [UniProt# Q9NUX5]

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: monkey (95%), rat (82%), and mouse (72%).

Localization

Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

71.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit POT1 Antibody - BSA Free (NB500-176) is a polyclonal antibody validated for use in WB. Anti-POT1 Antibody: Cited in 18 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for POT1 Antibody - BSA Free

Western Blot: POT1 Antibody [NB500-176]

Western Blot: POT1 Antibody [NB500-176]

Western Blot: POT1 Antibody [NB500-176] - Detection of POT1 in HeLa nuclear extracts (25 ug) using NB500-176(1:500). ECL detection 1 min.
Western Blot: POT1 Antibody [NB500-176]

Western Blot: POT1 Antibody [NB500-176]

POT1-Antibody-Western-Blot-NB500-176-img0004.jpg
POT1 Antibody

Western Blot: POT1 Antibody [NB500-176] -

Widespread dysregulation of shelterin protein expression in bortezomib-treated HEL & BGC-823 cellsA. & B. mRNA levels of shelterin factors TRF1, TRF2, TPP1, POT1, RAP1 & TIN2 in bortezomib-treated cells. Cells were treated with bortezomib for 24 hours & qPCR was used for quantitative assays. The levels of each target mRNA in bortezomib-treated cells were expressed as percentages of those in untreated cells. (A) HEL cells & (B) BGC-823 cells. C. & D. Immunoblotting assessment of TRF1, TRF2 & POT1 protein expression in bortezomib-treated cells. Same sets of cells above were analyzed for TRF1, TRF2 & POT1 protein levels & shown was representative of three independent experiments. (C) HEL cells & (D) BGC-823 cells. * & **: P < 0.05 & 0.01, respectively. BTZ, bortezomib. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.5752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
POT1 Antibody

Western Blot: POT1 Antibody [NB500-176] -

Attenuation of bortezomib-induced shelterin protein dysregulation by hTERT over-expressionA. & B. Cells expressing ectopic hTERT were treated with bortezomib for 24 hours & mRNA levels of shelterin proteins then analyzed using qPCR. The levels of each target mRNA in bortezomib-treated cells were expressed as percentages of those in untreated cells. (A) HEL-hTERT cells & (B) BGC-823-hTERT cells. C. & D. Immunoblotting assessment of TRF1, TRF2 & POT1 protein expression in bortezomib-treated cells. Same sets of cells above were analyzed for TRF1, TRF2 & POT1 protein levels & shown was representative of three independent experiments. (C) HEL-hTERT cells & (D) BGC-823-hTERT cells.* & **: P < 0.05 & 0.01, respectively. BTZ, bortezomib. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.5752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
POT1 Antibody

Western Blot: POT1 Antibody [NB500-176] -

Widespread dysregulation of shelterin protein expression in bortezomib-treated HEL & BGC-823 cellsA. & B. mRNA levels of shelterin factors TRF1, TRF2, TPP1, POT1, RAP1 & TIN2 in bortezomib-treated cells. Cells were treated with bortezomib for 24 hours & qPCR was used for quantitative assays. The levels of each target mRNA in bortezomib-treated cells were expressed as percentages of those in untreated cells. (A) HEL cells & (B) BGC-823 cells. C. & D. Immunoblotting assessment of TRF1, TRF2 & POT1 protein expression in bortezomib-treated cells. Same sets of cells above were analyzed for TRF1, TRF2 & POT1 protein levels & shown was representative of three independent experiments. (C) HEL cells & (D) BGC-823 cells. * & **: P < 0.05 & 0.01, respectively. BTZ, bortezomib. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.5752), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for POT1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1 - 2 ug/ml
Application Notes
This POT1 antibody is use for Western blot, where a band at ~71.4 kDa is seen. Not recommended for ICC/IF. Nitrocellulose membranes may be necessary for optimal results, although this has not been completely determined. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 4 using NB500-176 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: POT1

POT1 (protection of telomeres protein 1), a member of telombin family, is expressed ubiquitously and localizes to nucleus/chromosomal telomeres. It forms a component of telomerase RNP (ribonucleoprotein) complex which is essential for chromosome termini replication. POT1 participates in shelterin complex/telosome (contains TERF1, TERF2, TINF2, TERF2IP, ACD and POT1 - regulates telomeric length alongwith protecting telomers), telomerase holoenzyme complex (TERT, DKC1, WRAP53/TCAB1, NOP10, NHP2, GAR1, TEP1, EST1A, POT1 and TERC) and is also found complexed with TERF1, TINF2 and TNKS1. POT1 interacts with TNKS1 and can heterodimerize with ACD for enhancing telomere elongation via increase in telomerase processivity. POT1 implicates in double-stranded telomeric DNA-binding TRF1 complex which is essential to the regulation of telomere length by cis-inhibition of telomerase. POT1 acts as a single-stranded telomeric DNA-binding protein and thus behaves as a downstream effector of TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. POT1 functional alterations can lead to G-strand overhang loss, chromosomal-end fusions, chromosomal rearrangements, and rapid cell cycle arrest, ultimately leading to senescence and apoptosis. Accordingly, POT1 expression is altered in tumors and is specifically upregulated in therapeutic/radiation-resistant cancer cells.

Long Name

Protection of Telomeres Protein 1

Alternate Names

DKFZp586D211, HPOT1, POT1 protection of telomeres 1 homolog, POT1-like telomere end-binding protein, protection of telomeres 1 homolog (S. pombe), protection of telomeres protein 1

Entrez Gene IDs

25913 (Human)

Gene Symbol

POT1

UniProt

Q9NUX5

Additional POT1 Products

Product Documents for POT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for POT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for POT1 Antibody - BSA Free

Customer Reviews for POT1 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human lympoblast cells, Sample Amount: 35 ug
    Species: Human
    Verified Customer | Posted 09/23/2009

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Protocols

View specific protocols for POT1 Antibody - BSA Free (NB500-176):

Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturer's instructions.

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FAQs

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