PPAR gamma/NR1C3 Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-30473
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Key Product Details
Species Reactivity
Human, Mouse
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The epitope recognized by NBP3-30473 maps to a region between residue 50 to 100 of human Peroxisome Proliferator-Activated Receptor Gamma using the numbering given in entry NP_056953.2 (GeneID 5468).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for PPAR gamma/NR1C3 Antibody - BSA Free
Immunoprecipitation:[NBP3-30473]-
Immunoprecipitation:[NBP3-30473]-Detection of mouse PPAR-gamma by western blot of immunoprecipitates. Samples: Whole cell lysate from differentiated mouse NIH 3T3-L1 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. PPAR-gamma was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated PPAR-gamma, was used at 0. 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
PPAR gamma/NR1C3 Antibody - BSA Free NBP3-30473]-
Whole cell lysate (50 µg) from HeLa, HEK293T,Jurkat, differentiated mouse NIH 3T3-L1, and mouseNIH3T3-L1 cells prepared using NETN lysis buffer.Antibodies: Affinity purified rabbit anti-PPAR-gammaantibody used for WB at0.04 µg/ml. Detection: Chemiluminescence with anexposure time of 75 seconds
PPAR gamma/NR1C3 Antibody - BSA Free-NBP3-30473-
Whole cell lysate (50 µg) from U937 cellsincubated with (+) or without (-) TPA (200nM, 72 hrs) andlysed using NETN lysis buffer. Antibodies: Affinity purifiedrabbit anti-PPAR-gamma antibody used for WB at 0.1 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.Applications for PPAR gamma/NR1C3 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 µg/mg lysate
Western Blot
1:10,000 - 1:25,000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
Tris-citrate/phosphate buffer, pH 7 to 8
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: PPAR gamma/NR1C3
Long Name
Peroxisome Proliferator-activated Receptor gamma
Alternate Names
NR1C3, PPARG
Gene Symbol
PPARG
Additional PPAR gamma/NR1C3 Products
Product Documents for PPAR gamma/NR1C3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for PPAR gamma/NR1C3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
TGF-beta Signaling Pathways
