PPP3CB Antibody (5D3) - Azide and BSA Free

Novus Biologicals | Catalog # H00005532-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5D3

Format

Azide and BSA Free
View all PPP3CB Primary Antibodies »

Product Specifications

Immunogen

PPP3CB (NP_066955, 435 a.a. ~ 524 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LTPTGMLPSGVLAGGRQTLQSATVEAIEAEKAIRGFSPPHRICSFEEAKGLDRINERMPPRKDAVQQDGFNSLNTAHATENHGTGNHTAQ

Specificity

PPP3CB - protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform (calcineurin A beta)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PPP3CB Antibody (5D3) - Azide and BSA Free

Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]

Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]

Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB expression in transfected 293T cell line by PPP3CB monoclonal antibody (M01), clone 5D3.Lane 1: PPP3CB transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]

Western Blot: PPP3CB Antibody (5D3) [H00005532-M01]

Western Blot: PPP3CB Antibody (5D3) [H00005532-M01] - Analysis of PPP3CB over-expressed 293 cell line, cotransfected with PPP3CB Validated Chimera RNAi ( Cat # H00005532-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PPP3CB monoclonal antibody (M01), clone 5D3 (Cat # H00005532-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: PPP3CB Antibody (5D3) [H00005532-M01] - Detection limit for recombinant GST tagged PPP3CB is approximately 0.03ng/ml as a capture antibody.

Applications for PPP3CB Antibody (5D3) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PPP3CB

Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin

Long Name

Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform

Alternate Names

CALNA2, CALNB, CNA beta, CNA2

Entrez Gene IDs

5532 (Human)

Gene Symbol

PPP3CB

OMIM

114106 (Human)

UniProt

NP_066955 (Human)

Additional PPP3CB Products

Product Documents for PPP3CB Antibody (5D3) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PPP3CB Antibody (5D3) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs

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