PRAME Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-23670

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (%), Equine (%), Primate (%). Backed by our 100% Guarantee.

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PRAME Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acid 460-509 of human PRAME was used as the immunogen for the antibody.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: chimpanzee (92%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for PRAME Antibody - BSA Free

Western Blot: PRAME Antibody [NBP2-23670]

Western Blot: PRAME Antibody [NBP2-23670]

Western Blot: PRAME Antibody [NBP2-23670] - Analysis of MCF7 lysate using 1) pre-immune sera and 2) PRAME antibody at 1:5000 dilution.
Simple Western: PRAME Antibody [NBP2-23670]

Simple Western: PRAME Antibody [NBP2-23670]

Simple Western: PRAME Antibody [NBP2-23670] - Simple Western lane view shows a specific band for PRAME in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for PRAME Antibody - BSA Free

Application
Recommended Usage

Simple Western

1:5000

Western Blot

1:1000-1:5000
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MCF-7 lysate 0.2 mg/mL, separated by Size, antibody dilution of 1:5000, apparent MW was 59 kDa.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole sera with 50% Glycerol

Format

BSA Free

Preservative

0.025% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRAME

PRAME encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Recent data from Epping et.al., (Cell, Vol. 122: 835) suggest that overexpression of PRAME frequently observed in human cancers confers growth or survival advantages by antagonizing RAR signaling.

Alternate Names

cancer/testis antigen 130, MAPEOIP4CT130, melanoma antigen preferentially expressed in tumors, OIP-4, Opa-interacting protein 4, Opa-interacting protein OIP4, preferentially expressed antigen in melanoma, Preferentially expressed antigen of melanoma

Gene Symbol

PRAME

UniProt

P78395 (Human)

Additional PRAME Products

Product Documents for PRAME Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for PRAME Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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