PRDM16/MEL1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] -

Immunocytochemistry/ Immunofluorescence: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunofluorescence of PRDM16/MEL1 in mouse brain tissue with PRDM16/MEL1 antibody at 20 u/ml.

Green: PRDM16/MEL1 Antibody
Blue: DAPI staining

Immunohistochemistry: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] -

Immunohistochemistry: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] - Immunohistochemistry of PRDM16/MEL1 in mouse brain tissue with PRDM16/MEL1 antibody at 2 u/ml.

Western Blot: PRDM16/MEL1 Antibody - BSA Free [NBP1-77096] -

Loss of Blimp-1 expression by Tregs increases adipocyte beiging in WAT.Male Foxp3-YFP-Cre+ (WT) and Blimp-1fl/fl mice crossed to Foxp3-YFP-Cre+ (CKO) were placed on SFD and analyzed at 26–28 weeks of age. (A) Bar graphs showing relative mRNA expression of the indicated gene from total iWAT, VAT, and BAT from 26- to 28-week-old SFD-fed WT and CKO mice. Values were normalized to beta -actin. (B) Western blots and bar graphs showing UCP1 and PRDM16 expression from total iWAT from SFD-fed WT and CKO mice. Each lane represents 1 biological replicate. beta -Actin loading control is shown. (C) Graph showing Ucp1 mRNA expression from in vitro differentiated beige adipocyte cell lines and treated with IL-10 for 48 hours. Each dot represents a technical replicate and is representative of 2 independent experiments. (D) Graph indicating energy expenditure (EE) in kcal per hour by WT and CKO mice on SFD. (E) Bar graph indicating rectal temperature in WT and CKO mice. Data from A, D, and E are presented as means +/- SEM and are from 2–3 independent experiments with 3–16 mice, where each dot represents 1 mouse and an unpaired 2-tailed Student’s t test or 1-way ANOVA was performed to determine significance. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33351782), licensed under a CC-BY license. Not internally tested by Novus Biologicals.