preQ₁-azide

Key Product Details

Product Description

preQ₁-azide is a modified analog of the natural substrate prequeosine1 (preQ1). It contains an azide handle allowing attachment of additional groups to preQ1 via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry. preQ₁-azide is a toolbox compound for RNA-TAG (transglycosylation at guanosine).

Other RNA-TAG toolbox compounds include preQ1-biotin (Cat. No. 7804) and preQ₁- alkyne (Cat. No. 7806)

Plasmids for expression of the E.coli TGT enzyme (#138201) and for cloning an RNA of interest into a vector containing the recognition motif at the 3' end (#138209) are available from Addgene.

Licensing Information

Sold under license from The Regents of the University of California.

Product Specifications for preQ₁-azide

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Preparing Stock Solutions for preQ₁-azide

The following data is based on the product molecular weight 419.49.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

1 Recalculate

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.38 mL	11.92 mL	23.84 mL
5 mM	0.48 mL	2.38 mL	4.77 mL
10 mM	0.24 mL	1.19 mL	2.38 mL
50 mM	0.05 mL	0.24 mL	0.48 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Zhang Site-specific and enzymatic cross-linking of sgRNA enables wavelength-selectable photoactivated control of CRISPR gene editing. J.Am.Chem.Soc. 2022 PMID: 35257575

Product Documents for preQ₁-azide