PRMT1 Antibody (PRMT1-171) - BSA Free

Novus Biologicals | Catalog # NB120-12189

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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Chicken, Hamster, Primate

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PRMT1-171

Format

BSA Free
View all PRMT1 Primary Antibodies »

Product Specifications

Immunogen

Monoclonal Anti-PRMT1 is derived from the PRMT1-171 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with full length PRMT1.

Reactivity Notes

Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

This reacts with PRMT1 and does not cross-react with PRMT2, 3, 4, and 5.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

~42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PRMT1 Antibody (PRMT1-171) - BSA Free

Western Blot: PRMT1 Antibody (PRMT1-171)BSA Free [NB120-12189]

Western Blot: PRMT1 Antibody (PRMT1-171)BSA Free [NB120-12189]

Western Blot: PRMT1 Antibody (PRMT1-171) - BSA Free [NB120-12189] - Cell line lysates were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-PRMT1 Clone: PRMT1-171. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. NRK 5. MDBK 6. MDCK 7. CHO 8. 3T3

Applications for PRMT1 Antibody (PRMT1-171) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a concentration of 2 ug/ml (determined by using a whole cell extract of the human kidney cell line 293T). Predicted molecular weight: 42 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PRMT1

PRMTs are a family of proteins that either monomethylate or dimethylate the guanidino nitrogen atoms of arginine side chains. This common post-translational modification is implicated in protein trafficking, signal transduction, and transcriptional regulation. Most methyltransferases use the methyl donor, S-adenosyl-L-methionine (AdoMet), as a cofactor.

Long Name

Protein Arginine Methyltransferase 1

Alternate Names

ANM1, HCP1, HMT1, HMT2, HRMT1L2, IR1B4

Entrez Gene IDs

3276 (Human); 15469 (Mouse); 60421 (Rat)

Gene Symbol

PRMT1

Additional PRMT1 Products

Product Documents for PRMT1 Antibody (PRMT1-171) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for PRMT1 Antibody (PRMT1-171) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Epigenetics

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