EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.
ProDots Recombinant Human EGF Protein
R&D Systems | Catalog # PRD236
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD236 has been discontinued.
View all EGF products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Applications
Bioactivity
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human EGF protein
Asn971 - Arg1023, with an N-terminal Met
Asn971 - Arg1023, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
6 kDa
SDS-PAGE
6 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.
The ED50 for this effect is 20-100 pg/mL.
Scientific Data Images for ProDots Recombinant Human EGF Protein
ProDots Recombinant Human EGF Protein Binding Activity
ProDots®Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED50 for this effect is 20-100 pg/mL.
ProDots Recombinant Human EGF Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa.Formulation, Preparation, and Storage
PRD236
| Reconstitution | For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: EGF
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
UniProt
Additional EGF Products
Product Documents for ProDots Recombinant Human EGF Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human EGF Protein
726219.1
For research use only
Related Research Areas
Citations for ProDots Recombinant Human EGF Protein
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