ProDots Recombinant Human EGF Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD236-10
PRD236-50
PRD236-01
ProDots Recombinant Human EGF Protein Binding Activity
2 Images
Product Details
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ProDots Recombinant Human EGF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.

The ED50 for this effect is 20-100 pg/mL.

Source
E. coli-derived human EGF protein
Asn971 - Arg1023, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
6 kDa
SDS-PAGE

6 kDa, reducing conditions

Product Datasheets

PRD236

Reconstitution

For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Binding Activity ProDots Recombinant Human EGF Protein Binding Activity View Larger

ProDots® Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED50 for this effect is 20-100 pg/mL.

SDS-PAGE ProDots Recombinant Human EGF Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name
Epidermal Growth Factor
Entrez Gene IDs
1950 (Human); 13645 (Mouse); 25313 (Rat)
Alternate Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone

FAQs

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