ProDots Recombinant Human EGF Protein

PRD236 Datasheet / COA / SDS

Supplied as ready-to-use 5 ug protein balls

Catalog #	Availability	Size / Price	Qty
PRD236-10
PRD236-50
PRD236-01

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ProDots Recombinant Human EGF Protein Binding Activity

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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

ProDots Recombinant Human EGF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

Benefits

Rolls out of bottle for easy cell culture medium preparation
Eliminates time spent aliquoting
One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

Activity

Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415.

The ED₅₀ for this effect is 20-100 pg/mL.

Source

E. coli-derived human EGF protein
Asn971 - Arg1023, with an N-terminal Met

Accession #

P01133

N-terminal Sequence
Analysis

Met

Predicted Molecular Mass

6 kDa

SDS-PAGE

6 kDa, reducing conditions

Product Datasheets

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD236

Reconstitution	For a stock solution, reconstitute at 500 μg/mL in sterile PBS, or simply roll ProDot^® directly into cell culture medium for immediate use.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Binding Activity

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ProDots^® Recombinant Human EGF stimulates cell proliferation of the Balb/3T3 mouse embryonic fibroblast cell line.The ED₅₀ for this effect is 20-100 pg/mL.

SDS-PAGE

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1 μg/lane of ProDots^® Recombinant Human EGF was resolved with SDS-PAGE under reducing (R) conditions and visualized with silver staining, showing a single band at 6 kDa.

Reconstitution Calculator

Background: EGF

EGF (Epidermal Growth Factor) is expressed as a transmembrane protein that is proteolytically cleaved to generate soluble forms. It signals through EGF R/ErbB1 which heterodimerizes with ErbB2, ErbB3, or ErbB4 and can also associate with PDGF R beta and HGF R/c-MET. During development, EGF regulates thymocyte differentiation, neuroglia production, and adipocyte maturation. In the adult, EGF plays a role in mammary gland lactogenesis, fibroblast mitosis, dissociation of the extracellular matrix, and cell migration.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone