FGF-10 (Fibroblast Growth Factor 10) is a heparin binding glycoprotein in the subgroup of FGFs that also includes FGF-3, -7, and -22. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP. It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb). The mitogenic and chemotactic properties of FGF-10 are critical in limb bud initiation, palate development, branching morphogenesis and directional outgrowth of lung buds, formation of the otic vesicle and chochlea, adipogenesis, and the development of prostate, mammary, lacrimal, and submandibular salivary glands. The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung. Overlapping expression patterns and activities with FGF-3, -7, and -8 suggest at least a partial redundancy in FGF-10 biology. FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer.
ProDots Recombinant Human FGF-10 Protein
R&D Systems | Catalog # PRD345
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
Accession Number
Applications
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
Cys37 - Ser208 & Gly41 - Ser208
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 20-100 ng/mL.
Scientific Data Images for ProDots Recombinant Human FGF-10 Protein
ProDots Recombinant Human FGF-10 Protein Bioactivity
ProDots®Recombinant Human FGF-10 stimulated cell proliferation of the 4MBr-5 rhesus monkey epithelial cell line. The ED50 for this effect is 20-100 ng/mL.
ProDots Recombinant Human FGF-10 Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 21 kDa.Formulation, Preparation, and Storage
PRD345
| Reconstitution | For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: FGF-10
Long Name
Alternate Names
Gene Symbol
UniProt
Additional FGF-10 Products
Product Documents for ProDots Recombinant Human FGF-10 Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human FGF-10 Protein
726884.0
For research use only
Related Research Areas
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