FGF basic/FGF2/bFGF is a growth factor that functions in angiogenesis, wound healing, tissue repair, learning and memory, and the morphogenesis of heart, bone, and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic/FGF2/bFGF binds to FGFR1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin, Integrin alpha V beta 3, soluble FGFR1, FGF-BP, free gangliosides, Thrombospondin, Pentraxin 3/TSG-14, Fibrinogen, alpha 2-Macroglobulin, PDGF, and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners, extracellular matrix decoys or reservoirs, and soluble scavengers or chaperones. In particular, the interaction of FGF basic/FGF2/bFGF with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors.
ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein
R&D Systems | Catalog # PRD233
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
Accession Number
Applications
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
Pro143 - Ser288
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
17 kDa, reducing conditions
Activity
The ED50 for this effect is 0.1-0.6 ng/mL.
Scientific Data Images for ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein
ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein Bioactivity
ProDots®Recombinant Human FGF basic/FGF2 stimulates cell proliferation of the NR6R-3T3 mouse fibroblast cell line. The ED50 for this effect is 0.1-0.6 ng/mL.
ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human FGF basic/FGF2 was resolved with SDS-PAGE under reducing (R) and non-reducing(NR) conditions and visualized with silver staining, showing a single band at 17 kDa.Formulation, Preparation, and Storage
PRD233
| Reconstitution | For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual
defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: FGF basic/FGF2/bFGF
Long Name
Alternate Names
Gene Symbol
UniProt
Additional FGF basic/FGF2/bFGF Products
Product Documents for ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein
726190.4
For research use only
Related Research Areas
Customer Reviews for ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein (1)
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Verified Customer | Posted 05/17/2016Excellent product. Very easy to resuspend and use.
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FAQs for ProDots Recombinant Human FGF basic/FGF2 (146 aa) Protein
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Q: Does human FGF basic show activity on mouse cells?
A: Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.
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Q: What receptors does FGF basic bind?
A: FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors
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Q: Does human FGF basic show activity on mouse cells?
A: Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.
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Q: What receptors does FGF basic bind?
A: FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors
