ProDots Recombinant Human IL-10 Protein Summary
Pre-aliquoted, lyophilized protein dots for use in cell culture mediumFeatures and Benefits
- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Ser19 - Asn178
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Reconstitution||For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use.
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.
ProDots®Recombinant Human IL-10 induces proliferation of a MC/9-2 mouse mast cell line. The ED50for this effect is 0.15-0.75 ng/mL.
1 μg/lane of ProDots®Recombinant Human IL-10 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 18 kDa and 16 kDa, respectively.
Interleukin-10 functions as an anti-inflammatory cytokine by inhibiting the expansion and activation of Th1 cells and Th17 cells and by promoting the development of M2 macrophages and regulatory T cells (Treg). Within a tumor microrenvironment, however, IL-10 can inhibit the expansion of both Treg and myeloid-derived suppressor cells (MDSC). IL-10 exerts protective effects including limiting tissue damage in arthritic inflammation and promoting muscle regeneration after injury, but it also contributes to the persistence of viral infections. IL-10 signals through a receptor complex composed of IL-10 R alpha and IL-10 R beta. IL-10 R beta additionally associates with IL-20 R alpha, IL-22 R alpha 1, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29.
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