M-CSF (CSF-1) is the primary regulator of macrophage survival, proliferation and differentiation. It is essential for macrophage-related functions such as bone resorption, vascular development, and innate immunity. It signals through M-CSF R on monocytes, tissue macrophages, and osteoblasts. Both M-CSF and its receptor can be expressed by tumor cells, allowing M-CSF to act as an autocrine growth factor for cancer cells. Full length M-CSF is expressed as a transmembrane dimer, but various additional forms can be generated by alternative splicing, differential glycosylation, chondroitin sulfate proteoglycan modification, and proteolytic shedding.
ProDots Recombinant Human M-CSF Protein
R&D Systems | Catalog # PRD216
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD216 has been discontinued.
View all M-CSF products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Applications
Bioactivity
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human M-CSF protein
Glu33 - Ser190, with an N-terminal Met
Glu33 - Ser190, with an N-terminal Met
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
18.5 kDa (monomer)
Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860.
The ED50 for this effect is 0.5-1.5 ng/mL.
The ED50 for this effect is 0.5-1.5 ng/mL.
Scientific Data Images for ProDots Recombinant Human M-CSF Protein
ProDots Recombinant Human M-CSF Protein Bioactivity
ProDots®Recombinant Human M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner. The ED50 for this effect is 0.5-1.5 ng/mL.
ProDots Recombinant Human M-CSF Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human M-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing bands at 19 kDa and 35 kDa, respectively.Formulation, Preparation, and Storage
PRD216
| Reconstitution | For a stock solution, reconstitute at 50-500 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: M-CSF
Long Name
Macrophage Colony Stimulating Factor
Alternate Names
CSF-1, CSF1, Lanimostim, MCSF
Gene Symbol
CSF1
UniProt
Additional M-CSF Products
Product Documents for ProDots Recombinant Human M-CSF Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human M-CSF Protein
726881.0
For research use only
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