ProDots Recombinant Human M-CSF Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD216-10
PRD216-50
PRD216-01
ProDots Recombinant Human M-CSF Protein Bioactivity
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ProDots Recombinant Human M-CSF Protein Summary

Pre-aliquoted, lyophilized protein dots for use in cell culture medium


Benefits
  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. Nakoinz, I. et al. (1990) J. Immunol. 145:860. The ED50 for this effect is 0.5-1.5 ng/mL.
Source
E. coli-derived human M-CSF protein
Glu33 - Ser190, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
18.5 kDa (monomer)

Product Datasheets

PRD216

Reconstitution For a stock solution, reconstitute at 50-500 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
 • 6 months from date of receipt at room temperature. 
 • 12 months from date of receipt at 2-8 °C as supplied. 
 • 1 month at 2-8 °C under sterile conditions after reconstitution. 
 • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

Bioactivity ProDots Recombinant Human M-CSF Protein Bioactivity View Larger

ProDots® Recombinant Human M-CSF stimulates cell proliferation of the M-NFS-60 mouse myelogenous luekemia lymphoblast cell line in a dose-dependent manner. The ED50 for this effect is
0.5-1.5 ng/mL.

SDS-PAGE ProDots Recombinant Human M-CSF Protein SDS-PAGE View Larger

1 μg/lane of ProDots® Recombinant Human M-CSF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing bands at 19 kDa and
35 kDa, respectively.

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Reconstitution Calculator

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Background: M-CSF

M-CSF, also known as CSF-1, is a four-alpha-helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. M-CSF is also essential for the survival and proliferation of osteoclast progenitors. M-CSF also primes and enhances macrophage killing of tumor cells and microorganisms, regulates the release of cytokines and other inflammatory modulators from macrophages, and stimulates pinocytosis. M-CSF increases during pregnancy to support implantation and growth of the decidua and placenta. Sources of M-CSF include fibroblasts, activated macrophages, endometrial secretory epithelium, bone marrow stromal cells and activated endothelial cells. The M-CSF receptor (c-fms) transduces its pleotropic effects and mediates its endocytosis. M-CSF mRNAs of various sizes occur. Full length human M-CSF transcripts encode a 522 amino acid (aa) type I transmembrane (TM) protein with a 464 aa extracellular region, a 21 aa TM domain, and a 37 aa cytoplasmic tail that forms a 140 kDa covalent dimer. Differential processing produces two proteolytically cleaved, secreted dimers. One is an N- and O- glycosylated 86 kDa dimer, while the other is modified by both glycosylation and chondroitin-sulfate proteoglycan (PG) to generate a 200 kDa subunit. Although PG-modified M-CSF can circulate, it may be immobilized by attachment to type V collagen. Shorter transcripts encode M-CSF that lack cleavage and PG sites and produce an N-glycosylated 68 kDa TM dimer and a slowly produced 44 kDa secreted dimer. Although forms may vary in activity and half-life, all contain the N-terminal 150 aa portion that is necessary and sufficient for interaction with the M-CSF receptor. The first 223 aa of mature human M-CSF shares 88%, 86%, 81% and 74% aa identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.

Long Name
Macrophage Colony Stimulating Factor
Entrez Gene IDs
1435 (Human); 12977 (Mouse)
Alternate Names
colony stimulating factor 1 (macrophage); CSF1; CSF-1; Lanimostim; macrophage colony stimulating factor; macrophage colony-stimulating factor 1; MCSF; M-CSF; MCSFlanimostim; MGC31930

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