ProDots Recombinant Human PDGF-BB Protein

Supplied as ready-to-use 5 ug protein balls

Pre-aliquoted, lyophilized protein dots for use in cell culture medium

  • Rolls out of bottle for easy cell culture medium preparation
  • Eliminates time spent aliquoting
  • One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium

Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?

ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.

What are ProDots® Proteins?

ProDots® proteins are R&D Systems industry-leading quality, highly bioactive proteins packaged into easy-to-use lyophilized “dots”. Designed to be stable at 4 ºC and to dissolve instantly, ProDots® proteins can be rolled into cell culture media to quickly produce media with a desired working protein concentration.

  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 1.5-6.0 ng/mL.
  • Source
    E. coli-derived Ser82 - Thr190
  • Accession #
  • N-terminal Sequence
  • Predicted Molecular Mass
    12.3 (monomer) kDa
    12 kDa, reducing conditions
Reconstitution Add ProDot directly to medium or reconstitute at 100 µg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thawcycles.
• 6 months from date of receipt at 2-8 °C assupplied.
• 1 month at 2-8 °C under sterile conditions afterreconstitution.
• 3 months at -20 to -80 °C under sterile conditionsafter reconstitution.
Data Images
1 μg/lane of ProDots® Recombinant Human PDF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.
ProDots® Recombinant Human PDGF-BB stimulates cell proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is typically 1.5-6.0 ng/mL.
Background: PDGF-BB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
  • Long Name:
    Platelet-derived Growth Factor BB
  • Entrez Gene IDs:
    5155 (Human); 24628 (Rat)
  • Alternate Names:
Related Research Areas

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