PDGF-BB (Platelet-Derived Growth Factor BB) is a homodimeric molecule expressed by hepatocytes and nonresorbing osteoclasts, and it promotes osteoblast development and bone formation. It is also produced by platelets, macrophages, and mast cells. At sites of injury, it promotes neutrophil and macrophage infiltration for debridement, fibroblast secretion of new extracellular matrix, and IGF-I-mediated re-epithelialization. The traditional receptor for PDGF is either a homodimer or heterodimer created from two type I transmembrane RTKs, PDGF R alpha and PDGF R beta. PDGF-BB has been shown to bind the alpha/alpha homodimer, alpha/beta heterodimer, and the beta/beta homodimer in vitro, and act through the beta/beta homodimer in vivo.
ProDots Recombinant Human PDGF-BB Protein
R&D Systems | Catalog # PRD220
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD220 has been discontinued.
View all PDGF-BB products.
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
E. coli
Accession Number
Structure / Form
Disulfide-linked homodimer
Applications
Bioactivity
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Why use ProDots™ Proteins Instead of Standard Lyophilized Protein?
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
E. coli-derived human PDGF-BB protein
Ser82 - Thr190
Ser82 - Thr190
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ser82
Predicted Molecular Mass
12.3 kDa (monomer)
SDS-PAGE
12 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749.
The ED50 for this effect is 1.5-6.0 ng/mL.
The ED50 for this effect is 1.5-6.0 ng/mL.
Scientific Data Images for ProDots Recombinant Human PDGF-BB Protein
ProDots Recombinant Human PDGF-BB Protein Bioactivity
ProDots®Recombinant Human PDGF-BB stimulates cell proliferation in the NR6R-3T3 mouse fibroblast cell line in a dose-dependent manner. The ED50 for this effect is 1.5-6.0 ng/mL.
ProDots Recombinant Human PDGF-BB Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human PDF-BB was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 13 kDa and 28 kDa, respectively.Formulation, Preparation, and Storage
PRD220
| Reconstitution | For a stock solution, reconstitute at 100 µg/mL in sterile 4
mM HCl, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: PDGF-BB
Long Name
Platelet-derived Growth Factor BB
Alternate Names
PDGFBB
Gene Symbol
PDGFB
UniProt
Additional PDGF-BB Products
Product Documents for ProDots Recombinant Human PDGF-BB Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human PDGF-BB Protein
726247.1
For research use only
Related Research Areas
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