ProDots Recombinant Human R-Spondin 1 Protein

Name: ProDots Recombinant Human R-Spondin 1 Protein
Brand: R&D Systems
SKU: PRD4645

R&D Systems | Catalog # PRD4645

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Product Documents

Supplied as ready-to-use 5 ug protein balls

Discontinued Product

PRD4645 has been discontinued. View all R-Spondin 1 products.

Product Specifications
Scientific Data
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Source

CHO

Accession Number

Q2MKA7

Applications

Bioactivity

View all R-Spondin 1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Arg31 - Ala263

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21 & Arg31

Predicted Molecular Mass

25.6 kDa

SDS-PAGE

39 kDa, reducing conditions

Activity

Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells.
The ED₅₀ for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Images for ProDots Recombinant Human R-Spondin 1 Protein

ProDots Recombinant Human R-Spondin 1 Protein SDS-PAGE

1 μg/lane of ProDots^®Recombinant Human R-Spondin 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 39 kDa.

ProDots Recombinant Human R-Spondin 1 Protein Bioactivity

ProDots^®Recombinant Human R-Spondin 1, in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay.

Formulation, Preparation, and Storage

PRD4645

Reconstitution	For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll ProDot^® directly into cell culture medium.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: R-Spondin 1

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/beta-catenin signaling regulators. R-Spondin 1 enhances canonical Wnt/beta-catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors. R-Spondin 1 functions in dorsal neural tube development as well as male and female germ cell development. It also induces bone formation, intestinal crypt cell proliferation, angiogenesis, and insulin secretion from pancreatic beta cells.