ProDots Recombinant Human R-Spondin 1 Protein

Supplied as ready-to-use 5 ug protein balls
Catalog # Availability Size / Price Qty
PRD4645-10
PRD4645-50
PRD4645-01
ProDots Recombinant Human R-Spondin 1 Protein SDS-PAGE
2 Images
Product Details
FAQs
Supplemental Products
Reviews

ProDots Recombinant Human R-Spondin 1 Protein Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 1.0-4.0 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Source
Chinese Hamster Ovary cell line, CHO-derived human R-Spondin 1 protein
Arg31 - Ala263
Accession #
N-terminal Sequence
Analysis
Ser21 & Arg31
Predicted Molecular Mass
25.6 kDa
SDS-PAGE
39 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

PRD4645

Reconstitution For a stock solution, reconstitute at 100 μg/mL in sterile PBS. For immediate use, simply roll ProDot® directly into cell culture medium.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• 6 months from date of receipt at room temperature as supplied.
• 12 months from date of receipt at 2-8 °C as supplied.
• 1 month at 2-8 °C under sterile conditions after reconstitution.
• 3 months at -20 to -80 °C under sterile conditions after reconstitution.

Data Images

SDS-PAGE View Larger

1 μg/lane of ProDots®Recombinant Human R-Spondin 1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 39 kDa.

Bioactivity View Larger

ProDots®Recombinant Human R-Spondin 1, in the presence of Recombinant Mouse Wnt-3a (Catalog # 1324-WN; 5 ng/mL), induces activation of beta-catenin in HEK293T cells measured using the Topflash assay.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: R-Spondin 1

R-Spondin 1 (RSPO1), also known as Cristin 3, is a 27 kDa secreted protein in the R-Spondin family of Wnt/beta-catenin signaling regulators. R-Spondin 1 enhances canonical Wnt/beta-catenin signaling by competing with the Wnt antagonist Dkk-1, binding to Frizzled-8, Kremen, LRP-6, Lgr4, Lgr5, and Lgr6, and enhancing cell surface availability of Wnt receptors. R-Spondin 1 functions in dorsal neural tube development as well as male and female germ cell development. It also induces bone formation, intestinal crypt cell proliferation, angiogenesis, and insulin secretion from pancreatic beta cells.

Long Name
Roof Plate-specific Spondin 1
Entrez Gene IDs
284654 (Human); 192199 (Mouse)
Alternate Names
Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; HRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Animal-Free Recombinant Proteins

GMP Recombinant Proteins

ProDots Proteins

Recombinant Proteins

Reconstitution Buffers

Tocris Peptides

Tocris Small Molecules

Reviews for ProDots Recombinant Human R-Spondin 1 Protein

There are currently no reviews for this product. Be the first to review ProDots Recombinant Human R-Spondin 1 Protein and earn rewards!

Have you used ProDots Recombinant Human R-Spondin 1 Protein?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review