SCF (Stem cell factor), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF) promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. SCF is expressed as a transmembrane, but a soluble form can be released by proteolytic shedding. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
ProDots Recombinant Human SCF/c-kit Ligand Protein
R&D Systems | Catalog # PRD255
Key Product Details
Pre-aliquoted, lyophilized protein dots for use in cell culture medium
Features and Benefits- Rolls out of bottle for easy cell culture medium preparation
- Eliminates time spent aliquoting
- One protein dot can be used to make 500 mL of 10 ng/mL cell culture medium
Source
Accession Number
Applications
ProDots® proteins are designed to reduce cell culture media preparation time while ensuring optimal protein recovery and bioactivity. Unlike standard lyophilized protein, which is shipped as a diffuse residue within a vial, ProDots® proteins are neatly bundled into the shape of a ball. In this form ProDots® proteins can be rolled directly into cell culture media. ProDots® proteins rehydrate instantly in media to maximize the recovery of purchased protein and to reliably reconstitute it at a desired concentration for use in cell culture media. ProDots® proteins can be conveniently stored at room temperature where they will remain stable for 6 months or at 4 ºC where they will remain stable for 12 months. The accessible storage and high stability of ProDots® proteins makes them ideal for quick and easy preparation of cell culture media.
Product Specifications
Source
Glu26-Ala189, with an N-terminal Met
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The ED50 for this effect is 1-5 ng/mL.
Scientific Data Images for ProDots Recombinant Human SCF/c-kit Ligand Protein
ProDots Recombinant Human SCF/c-kit Ligand Protein Bioactivity
ProDots®Recombinant Human SCF stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 1-5 ng/mL.
ProDots Recombinant Human SCF/c-kit Ligand Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Human SCF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 19 kDa.Formulation, Preparation, and Storage
PRD255
| Reconstitution | For a stock solution, reconstitute at 50 μg/mL in sterile PBS, or simply roll ProDot® directly into cell culture medium for immediate use. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: SCF/c-kit Ligand
Long Name
Alternate Names
Gene Symbol
UniProt
Additional SCF/c-kit Ligand Products
Product Documents for ProDots Recombinant Human SCF/c-kit Ligand Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Human SCF/c-kit Ligand Protein
726192.5
For research use only
Related Research Areas
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