LIF (Leukemia Inhibitory Factor) is a pleiotropic member of the IL-6 family of cytokines. It functions through a heterodimeric receptor complex containing a ligand-binding subunit, LIF R alpha/CD118, and a signal transducing subunit, gp130. gp130 also serves as a subunit of the receptor complexes for Oncostatin M, Cardiotrophin-1, CNTF, IL-6, IL-11, and IL-27. LIF supports uterine implantation of the embryo, pluripotency in embryonic stem cells, and proliferation of progenitor cells. It promotes regulatory T cell and inhibits Th17 cell differentiation, and it is required by the thymic epithelium to support T cell maturation. It promotes motor neuron survival and oligodendrocyte myelination, enhances adrenal production of cortisol and aldosterone, and promotes differentiation of adipocytes and cardiac smooth muscle cells. LIF can also function as an autocrine growth factor in pancreatic cancer and can induce formation of immunosuppressive tumor-associated macrophages.
ProDots Recombinant Mouse LIF Protein
R&D Systems | Catalog # PRD449
Supplied as ready-to-use 5 ug protein balls
Discontinued Product
PRD449 has been discontinued.
View all LIF products.
Key Product Details
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived mouse LIF protein
Pro25 - Phe203
Pro25 - Phe203
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Pro25
Predicted Molecular Mass
20 kDa
SDS-PAGE
20 kDa, reducing conditions
Activity
Measured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells.
The ED50 is 0.1-0.6 ng/mL.
The ED50 is 0.1-0.6 ng/mL.
Scientific Data Images for ProDots Recombinant Mouse LIF Protein
ProDots Recombinant Mouse LIF Protein SDS-PAGE
1 μg/lane of ProDots®Recombinant Mouse LIF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized with silver staining, showing a single band at 20 kDa.
ProDots Recombinant Mouse LIF Protein Bioactivity
ProDots®Recombinant Mouse LIF induces IL-6 secretion in M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.1-0.6 ng/mL.Formulation, Preparation, and Storage
PRD449
| Reconstitution | For a stock solution, reconstitute at 0.2-1 mg/mL in sterile PBS. For immediate use, simply roll ProDot® directly into cell culture medium. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. • 6 months from date of receipt at room temperature as supplied. • 12 months from date of receipt at 2-8 °C as supplied. • 1 month at 2-8 °C under sterile conditions after reconstitution. • 3 months at -20 to -80 °C under sterile conditions after reconstitution. |
Calculators
Background: LIF
Long Name
Leukemia Inhibitory Factor
Alternate Names
D Factor, Emfilermin, HILDA, MLPLI
Gene Symbol
LIF
UniProt
Additional LIF Products
Product Documents for ProDots Recombinant Mouse LIF Protein
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for ProDots Recombinant Mouse LIF Protein
727084.0
For research use only
Related Research Areas
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