Profilin 1 [p Tyr129] Antibody

Novus Biologicals | Catalog # NBP3-23345

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Profilin 1 Primary Antibodies »

Product Specifications

Immunogen

Phospho-Profilin (Tyr-129) synthetic peptide (coupled to carrier protein) that includes amino acids surrounding Tyr-129 in human Profilin 1. This sequence is well conserved in rat and mouse Profilin 1. The tyrosine site has some homology to the conserved site (Tyr-129) in Profilin-2a and Profilin-2b, but the site is not conserved in Profiin-3 and Profilin-4. [UniProt# P07737]

Modification

p Tyr129

Specificity

This antibody was cross-adsorbed to unphosphorylated Profilin 1 (Tyr-129) then affinity purified using Profilin 1 (Tyr-129) peptide (without carrier). The antibody detects a 15 kDa band corresponding to the apparent molecular mass of Profilin 1 in human umbilical vein endothelial cells (HUVEC) and jurkat cells treated with pervanadate. This reactivity is removed by alkaline phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for Profilin 1 [p Tyr129] Antibody

Profilin 1 [p Tyr129] Antibody

Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] -

Western Blot: Profilin 1 [p Tyr129] Antibody [NBP3-23345] - Western blot of HUVEC stimulated with Pervanadate (1 mM) for 30 min (lanes 1-4). The blots were treated with alkaline phosphatase to remove phosphorylation (lanes 2 & 4), then probed with anti-Profilin (a.a. 126-137) (lanes 1 & 2) or anti-Profilin (Tyr-129) phospho-specific (lanes 3 & 4). The antibodies detect profilin at 15 kDa. In lanes 3 & 4, the antibody also detects an unknown 20 kDa protein.

Applications for Profilin 1 [p Tyr129] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Profilin 1

Profilin is an ubiquitous small (12-15 kDa) phosphoinositides and poly-L-proline binding protein that plays a role both in signal transduction pathways and actin filament dynamics. There are two mammalian profilins with similar biochemical properties but different expression patterns. Whereas profilin I appears to be highly expressed in most tissues except for skeletal muscle, profilin II is predominantly expressed in brain and at lower levels. It is also in expressed in skeletal muscle, the uterus and kidneys. Profilin is a mainly cytosolic protein with higher concentrations in dynamic membrane areas like the leading edge and ruffling membranes.

Alternate Names

PFN1, PROF1, Profilin I

Gene Symbol

PFN1

Additional Profilin 1 Products

Product Documents for Profilin 1 [p Tyr129] Antibody

Certificate of Analysis

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Product Specific Notices for Profilin 1 [p Tyr129] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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