Proteasome 19S 10B Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-99616

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Proteasome 19S 10B Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human Proteasome 19S 10B.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Proteasome 19S 10B Antibody - BSA Free

Western Blot: Proteasome 19S 10B Antibody [NBP2-99616]

Western Blot: Proteasome 19S 10B Antibody [NBP2-99616]

Western Blot: Proteasome 19S 10B Antibody [NBP2-99616] - Anti-Proteasome 19S 10B rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa
Immunoprecipitation: Proteasome 19S 10B Antibody [NBP2-99616]

Immunoprecipitation: Proteasome 19S 10B Antibody [NBP2-99616]

Immunoprecipitation: Proteasome 19S 10B Antibody [NBP2-99616] - Proteasome 19S 10B was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 ul anti-Proteasome 19S 10B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Proteasome 19S 10B rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa. Observed band size: 100 kDa

Applications for Proteasome 19S 10B Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

PBS

Format

BSA Free

Preservative

0.03% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Proteasome 19S 10B

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multi-subunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome and Liddle syndrome.

Alternate Names

26S proteasome regulatory subunit RPN1, 26S proteasome regulatory subunit S2, MGC14274,55.11 protein, P97, proteasome (prosome, macropain) 26S subunit, non-ATPase, 2, Protein 55.11, Rpn1, S226S proteasome non-ATPase regulatory subunit 2, TNFR-associated protein 2, TRAP226S proteasome subunit p97, tumor necrosis factor receptor-associated protein 2, Tumor necrosis factor type 1 receptor-associated protein 2

Gene Symbol

PSMD2

Additional Proteasome 19S 10B Products

Product Documents for Proteasome 19S 10B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Proteasome 19S 10B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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