Protein A Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB99762G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB99762G-100UG

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Protein A Alexa Fluor® 488-conjugated Antibody Summary

Specificity
Detects Protein A in direct ELISAs.
Source
Monoclonal Rat IgG2a Clone # 946425
Purification
Protein A or G purified
Immunogen
Recombinant Protein A
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
ELISA
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Protein A

The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is used in purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.

Entrez Gene IDs
3919448 (S. aureus)
Alternate Names
Immunoglobulin G binding protein A; PROA; Protein A; Protein-A; SPA; Staphylococcal protein A

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