Proteome Profiler Human Adipokine Array Kit ARY024: R&D Systems
Kit Summary

To simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary.

 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.

 

 
Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin FGF basic M-CSF
Angiopoietin-1 FGF-19 MIF
Angiopoietin-2 Fibrinogen Myeloperoxidase
Angiopoietin-like 2 Growth Hormone Nidogen-1/Entactin
Angiopoietin-like 3 HGF Oncostatin M
BAFF/BLyS ICAM-I Pappalysin-1/PAPP-A
BMP-4 IGFBP-2 PBEF/Visfatin
Cathepsin D IGFBP-3 PCSK9
Cathepsin L IGFBP-4 Pentraxin-3
Cathepsin S IGFBP-6 Pref-1/DLK-1/FA1
CCL2/MCP-1 IGFBP-rp1/IGFBP-7 RAGE
CCL5/RANTES IL-1 beta Resistin
Chemerin IL-6 Serpin A12
Complement Factor D IL-10 Serpin A8/AGT
C-Reactive Protein IL-11 Serpin E1/PAI-1
CXCL8/IL-8 LAP (TGF-ß1) TIMP-1
DPPIV/CD26 Leptin TIMP-3
Endocan LIF TNF-alpha
EN-RAGE Lipocalin-2/NGAL VEGF
Fetuin B    
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria
    Authors: N Suffee, T Moore-Morr, P Farahmand, C Rcker-Mar, G Dilanian, M Fradet, D Sawaki, G Derumeaux, P LePrince, K Cl‚ment, I Dugail, M Puceat, SN Hatem
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017

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