Proteome Profiler Human Adipokine Array Kit

Contains 4 membranes-each spotted in duplicate with 58 different obesity-related antibodies
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R&D Systems Proteome Profiler Antibody Array Kits
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Product Details
Citations (7)

Proteome Profiler Human Adipokine Array Kit Summary

Kit Summary

To simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary.


General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.


Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.


Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin FGF basic M-CSF
Angiopoietin-1 FGF-19 MIF
Angiopoietin-2 Fibrinogen Myeloperoxidase
Angiopoietin-like 2 Growth Hormone Nidogen-1/Entactin
Angiopoietin-like 3 HGF Oncostatin M
BAFF/BLyS ICAM-I Pappalysin-1/PAPP-A
BMP-4 IGFBP-2 PBEF/Visfatin
Cathepsin D IGFBP-3 PCSK9
Cathepsin L IGFBP-4 Pentraxin-3
Cathepsin S IGFBP-6 Pref-1/DLK-1/FA1
CCL5/RANTES IL-1 beta Resistin
Chemerin IL-6 Serpin A12
Complement Factor D IL-10 Serpin A8/AGT
C-Reactive Protein IL-11 Serpin E1/PAI-1
DPPIV/CD26 Leptin TIMP-3
Endocan LIF TNF-alpha
Fetuin B    


Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

Citations for Proteome Profiler Human Adipokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Human Adipose Stem Cells (hASCs) Grown on Biodegradable Microcarriers in Serum- and Xeno-Free Medium Preserve Their Undifferentiated Status
    Authors: F Muoio, S Panella, V Jossen, M Lindner, Y Harder, M Müller, R Eibl, T Tallone
    Journal of functional biomaterials, 2021;12(2):.  2021
  2. Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells
    Authors: S Panella, F Muoio, V Jossen, Y Harder, R Eibl-Schin, T Tallone
    Cells, 2021;10(2):.  2021
  3. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019;45(0):511-518.  2019
  4. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019;45(0):511-518.  2019
  5. Breast-Associated Adipocytes Secretome Induce Fatty Acid Uptake and Invasiveness in Breast Cancer Cells via CD36 Independently of Body Mass Index, Menopausal Status and Mammary Density
    Authors: M Zaoui, M Morel, N Ferrand, S Fellahi, JP Bastard, A Lamazière, AK Larsen, V Béréziat, M Atlan, M Sabbah
    Cancers (Basel), 2019;11(12):.  2019
  6. Phenotyping of circulating extracellular vesicles (EVs) in obesity identifies large EVs as functional conveyors of Macrophage Migration Inhibitory Factor
    Authors: J Amosse, M Durcin, M Malloci, L Vergori, A Fleury, F Gagnadoux, S Dubois, G Simard, J Boursier, O Hue, MC Martinez, R Andriantsi, S Le Lay
    Mol Metab, 2018;18(0):134-142.  2018
  7. Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria
    Authors: N Suffee, T Moore-Morr, P Farahmand, C Rcker-Mar, G Dilanian, M Fradet, D Sawaki, G Derumeaux, P LePrince, K Cl‚ment, I Dugail, M Puceat, SN Hatem
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.  2017


  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs

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