Proteome Profiler Human Adipokine Array Kit
Proteome Profiler Human Adipokine Array Kit Summary
To simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary.
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Wash Buffer
- Detection Antibody Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.
Simultaneously detect the levels of these obesity-related proteins in a single sample. | ||
Adiponectin | FGF basic | M-CSF |
Angiopoietin-1 | FGF-19 | MIF |
Angiopoietin-2 | Fibrinogen | Myeloperoxidase |
Angiopoietin-like 2 | Growth Hormone | Nidogen-1/Entactin |
Angiopoietin-like 3 | HGF | Oncostatin M |
BAFF/BLyS | ICAM-I | Pappalysin-1/PAPP-A |
BMP-4 | IGFBP-2 | PBEF/Visfatin |
Cathepsin D | IGFBP-3 | PCSK9 |
Cathepsin L | IGFBP-4 | Pentraxin-3 |
Cathepsin S | IGFBP-6 | Pref-1/DLK-1/FA1 |
CCL2/MCP-1 | IGFBP-rp1/IGFBP-7 | RAGE |
CCL5/RANTES | IL-1 beta | Resistin |
Chemerin | IL-6 | Serpin A12 |
Complement Factor D | IL-10 | Serpin A8/AGT |
C-Reactive Protein | IL-11 | Serpin E1/PAI-1 |
CXCL8/IL-8 | LAP (TGF-ß1) | TIMP-1 |
DPPIV/CD26 | Leptin | TIMP-3 |
Endocan | LIF | TNF-alpha |
EN-RAGE | Lipocalin-2/NGAL | VEGF |
Fetuin B |
Specifications
Product Datasheets
Citations for Proteome Profiler Human Adipokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019;45(0):511-518. 2019
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Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019;45(0):511-518. 2019
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Breast-Associated Adipocytes Secretome Induce Fatty Acid Uptake and Invasiveness in Breast Cancer Cells via CD36 Independently of Body Mass Index, Menopausal Status and Mammary Density
Authors: M Zaoui, M Morel, N Ferrand, S Fellahi, JP Bastard, A Lamazière, AK Larsen, V Béréziat, M Atlan, M Sabbah
Cancers (Basel), 2019;11(12):. 2019
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Phenotyping of circulating extracellular vesicles (EVs) in obesity identifies large EVs as functional conveyors of Macrophage Migration Inhibitory Factor
Authors: J Amosse, M Durcin, M Malloci, L Vergori, A Fleury, F Gagnadoux, S Dubois, G Simard, J Boursier, O Hue, MC Martinez, R Andriantsi, S Le Lay
Mol Metab, 2018;18(0):134-142. 2018
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Atrial natriuretic peptide regulates adipose tissue accumulation in adult atria
Authors: N Suffee, T Moore-Morr, P Farahmand, C Rcker-Mar, G Dilanian, M Fradet, D Sawaki, G Derumeaux, P LePrince, K Cl‚ment, I Dugail, M Puceat, SN Hatem
Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):. 2017
FAQs
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Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?
The proteinase inhibitors are recommended for cell and tissue lysates only. You do not need them for cell culture supernates.
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