Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.
|Simultaneously detect the levels of these obesity-related proteins in a single sample.|
|Angiopoietin-like 2||Growth Hormone||Nidogen-1/Entactin|
|Angiopoietin-like 3||HGF||Oncostatin M|
|Complement Factor D||IL-10||Serpin A8/AGT|
|C-Reactive Protein||IL-11||Serpin E1/PAI-1|
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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