Proteome Profiler Human Adipokine Array Kit

Contains 4 membranes-each spotted in duplicate with 58 different obesity-related antibodies
Catalog # Availability Size / Price Qty
ARY024
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Proteome Profiler Human Adipokine Array Kit Summary

Kit Summary

To simultaneously detect the relative levels of 58 different obesity-related molecules in a single sample. No specialized equipment is necessary.

 

General Assay Principle

Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cell culture supernatant, cell lysate, tissue lysate, serum, plasma, saliva, urine, or human milk samples are diluted and mixed with a cocktail of biotinylated detection antibodies. The sample/antibody mixture is then incubated with the array. Any cytokine/detection antibody complex present is bound by its cognate immobilized capture antibody on the membrane. Streptavidin-Horseradish Peroxidase and chemiluminescent detection reagents are added, and a signal is produced in proportion to the amount of cytokine bound. Chemiluminescence is detected in the same manner as a Western blot.

 

Kit Contents
  • 4 Array Membranes
  • 4-Well Multi-dish
  • Array Buffers
  • Wash Buffer
  • Detection Antibody Cocktail
  • Streptavidin-HRP
  • Chemiluminescent Detection Reagents
  • Transparency Overlay Template
  • Detailed Protocol

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Reagents are stable for 12 months from date of receipt when stored in the dark at 2° C to 8°.

 

 
Simultaneously detect the levels of these obesity-related proteins in a single sample.
Adiponectin FGF basic M-CSF
Angiopoietin-1 FGF-19 MIF
Angiopoietin-2 Fibrinogen Myeloperoxidase
Angiopoietin-like 2 Growth Hormone Nidogen-1/Entactin
Angiopoietin-like 3 HGF Oncostatin M
BAFF/BLyS ICAM-I Pappalysin-1/PAPP-A
BMP-4 IGFBP-2 PBEF/Visfatin
Cathepsin D IGFBP-3 PCSK9
Cathepsin L IGFBP-4 Pentraxin-3
Cathepsin S IGFBP-6 Pref-1/DLK-1/FA1
CCL2/MCP-1 IGFBP-rp1/IGFBP-7 RAGE
CCL5/RANTES IL-1 beta Resistin
Chemerin IL-6 Serpin A12
Complement Factor D IL-10 Serpin A8/AGT
C-Reactive Protein IL-11 Serpin E1/PAI-1
CXCL8/IL-8 LAP (TGF-ß1) TIMP-1
DPPIV/CD26 Leptin TIMP-3
Endocan LIF TNF-alpha
EN-RAGE Lipocalin-2/NGAL VEGF
Fetuin B    

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

FAQs

No product specific FAQs exist for this product, however you may

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