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Proteome Profiler Human XL Cytokine Array Kit

ARY022B 77 Citations 14 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY022B
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
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Citations (77)
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Reviews (14)
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Proteome Profiler Human XL Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 105 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma CCL2/MCP-1
Angiogenin IGFBP-2 CCL7/MCP-3
Angiopoietin-1 IGFBP-3 M-CSF
Angiopoietin-2 IL-1 alpha/IL-1F1 MIF
Apolipoprotein A1 IL-1 beta/IL-1F2 CXCL9/MIG
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CCL3/CCL4 MIP-1 alpha/beta
BDNF IL-2 CCL20/MIP-3 alpha
CD14 IL-3 CCL19/MIP-3 beta
CD30 IL-4 MMP-9
CD31/PECAM-1 IL-5 Myeloperoxidase
CD40 Ligand/TNFSF5 IL-6 Osteopontin (OPN)
Chitinase 3-like IL-8 PDGF-AA
Complement Component C5/C5a IL-10 PDGF-AB/BB
Complement Factor D IL-11 Pentraxin 3/TSF-14
C-Reactive Protein/CRP IL-12 p70 CXCL4/PF4
Cripto-1 IL-13 RAGE
Cystatin C IL-15 CCL5/RANTES
Dkk-1 IL-16 RBP4
DPPIV/CD26 IL-17A Relaxin-2
EGF IL-18 BPa Resistin
CXCL5/ENA-78 IL-19 CXCL12/SDF-1 alpha
Endoglin/CD105 IL-22 Serpin E1/PAI-1
EMMPRIN IL-23 SHBG
Fas Ligand IL-24 ST2/IL1 R4
FGF basic IL-27 CCL17/TARC
KGF/FGF-7 IL-31 TFF3
FGF-19 IL-32 alpha/beta/gamma TfR
Flt-3 Ligand IL-33 TGF-alpha
G-CSF IL-34 Thrombospondin-1
GDF-15 CXCL10/IP-10 TIM-1
GM-CSF CXCL11/I-TAC TNF-alpha
CXCL1/GRO alpha Kallikrein 3/PSA uPAR
Growth Hormone (GH) Leptin VCAM-1
HGF LIF VEGF
ICAM-1/CD54 Lipocalin-2/NGAL Vitamin D BP

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Product Datasheets

Product Datasheet
COA
SDS

Data Example

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. View Larger

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

77 Citations: Showing 1 - 10
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  1. Isolation and Characterization of Human Synovial Fluid-Derived Mesenchymal Stromal Cells from Popliteal Cyst
    Authors: F Li, J Chen, M Gong, Y Bi, C Hu, Y Zhang, M Li
    Stem Cells Int, 2020;2020(0):7416493.  2020
  2. Anti-CD3 Antibody Treatment Reduces Scar Formation in a Rat Model of Myocardial Infarction
    Authors: B Wernly, V Paar, A Aigner, PM Pilz, BK Podesser, M Förster, C Jung, JP Hofbauer, B Tockner, M Wimmer, T Kraus, LJ Motloch, M Hackl, UC Hoppe, A Kiss, M Lichtenaue
    Cells, 2020;9(2):.  2020
  3. Reproducibility of GMP-compliant production of therapeutic stressed peripheral blood mononuclear cell-derived secretomes, a novel class of biological medicinal products
    Authors: M Laggner, A Gugerell, C Bachmann, H Hofbauer, V Vorstandle, M Seibold, G Gouya Lech, A Peterbauer, S Madlener, S Demyanets, D Sorgenfrey, T Ostler, M Erb, M Mildner, HJ Ankersmit
    Stem Cell Res Ther, 2020;11(1):9.  2020
  4. Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude
    Authors: A Gangwar, S Paul, Y Ahmad, K Bhargava
    Sci Rep, 2020;10(1):7899.  2020
  5. Impact of Type 2 Diabetes Mellitus on Human Bone Marrow Stromal Cell Number and Phenotypic Characteristics
    Authors: FC Cassidy, C Shortiss, CG Murphy, SR Kearns, W Curtin, C De Buitléi, T O'Brien, CM Coleman
    Int J Mol Sci, 2020;21(7):.  2020
  6. Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies
    Authors: S Carluccio, D Martinelli, MEF Palamà, RC Pereira, R Benelli, A Guijarro, R Cancedda, C Gentili
    Cells, 2020;9(4):.  2020
  7. Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration
    Authors: F Jiang, W Zhang, M Zhou, Z Zhou, M Shen, N Chen, X Jiang
    Theranostics, 2020;10(14):6216-6230.  2020
  8. Intrathecal Infusion of Autologous Adipose-Derived Regenerative Cells in Autoimmune Refractory Epilepsy: Evaluation of Safety and Efficacy
    Authors: E Szczepanik, H Mierzewska, D Antczak-Ma, A Figiel-Dab, I Terczynska, J Tryfon, N Krzesniak, BH Noszczyk, E Sawicka, K Domanska-J, A Sarnowska
    Stem Cells Int, 2020;2020(0):7104243.  2020
  9. TAK1 regulates the tumor microenvironment through inflammatory, angiogenetic and apoptotic signaling cascades
    Authors: SA Scarneo, KW Yang, JR Roques, A Dai, LS Eibschutz, P Hughes, TAJ Haystead
    Oncotarget, 2020;11(21):1961-1970.  2020
  10. Modulating Tumor-Associated Macrophage Polarization by Synthetic and Natural PPAR&gamma Ligands as a Potential Target in Breast Cancer
    Authors: G Gionfriddo, P Plastina, G Augimeri, S Catalano, C Giordano, I Barone, C Morelli, F Giordano, L Gelsomino, D Sisci, R Witkamp, S Andò, K van Norren, D Bonofiglio
    Cells, 2020;9(1):.  2020
  11. Permanent Pro-Tumorigenic Shift in Adipose Tissue-Derived Mesenchymal Stromal Cells Induced by Breast Malignancy
    Authors: J Plava, M Cihova, M Burikova, M Bohac, M Adamkov, S Drahosova, D Rusnakova, D Pindak, M Karaba, J Simo, M Mego, L Danisovic, L Kucerova, S Miklikova
    Cells, 2020;9(2):.  2020
  12. Nintedanib inhibits intrahepatic cholangiocarcinoma aggressiveness via suppression of cytokines extracted from activated cancer-associated fibroblasts
    Authors: T Yamanaka, N Harimoto, T Yokobori, R Muranushi, K Hoshino, K Hagiwara, D Gantumur, T Handa, N Ishii, M Tsukagoshi, T Igarashi, H Tanaka, A Watanabe, N Kubo, K Araki, K Shirabe
    Br. J. Cancer, 2020;0(0):.  2020
  13. SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer
    Authors: J Gwak, H Jeong, K Lee, JY Shin, T Sim, J Na, J Kim, BG Ju
    Cancers, 2020;12(9):.  2020
  14. Zoledronic Acid Abrogates Restraint Stress-Induced Macrophage Infiltration, PDGF-AA Expression, and Ovarian Cancer Growth
    Authors: CB Colon-Eche, T Ortiz, L Maldonado, MJ Hidalgo-Va, J Pérez-Mora, AN Aquino-Ace, R Herrera-No, M Bonilla-Cl, EM Castro, GN Armaiz-Pen
    Cancers, 2020;12(9):.  2020
  15. Vadadustat, a HIF Prolyl Hydroxylase Inhibitor, Improves Immunomodulatory Properties of Human Mesenchymal Stromal Cells
    Authors: K Zielniok, A Burdzinska, B Kaleta, R Zagozdzon, L Paczek
    Cells, 2020;9(11):.  2020
  16. Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells
    Authors: M Spirk, S Zimny, M Neumann, N McMullen, CJ Sinal, C Buechler
    Int J Mol Sci, 2020;21(20):.  2020
  17. Hypoxia Rapidly Induces the Expression of Cardiomyogenic Factors in Human Adipose-Derived Adherent Stromal Cells
    Authors: JW Choi, H Moon, SE Jung, S Lim, S Lee, IK Kim, HB Lee, J Lee, BW Song, SW Kim, KC Hwang
    J Clin Med, 2019;8(8):.  2019
  18. Blockage of TGF-? Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells
    Authors: Y Li, H Li, Y Duan, X Cai, D You, F Zhou, C Yang, X Tuo, Z Liu
    Biomed Res Int, 2019;2019(0):8231267.  2019
  19. Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature
    Authors: LS Spitzhorn, M Megges, W Wruck, MS Rahman, J Otte, Ö Degistiric, R Meisel, RV Sorg, ROC Oreffo, J Adjaye
    Stem Cell Res Ther, 2019;10(1):100.  2019
  20. p16INK4A-expressing mesenchymal stromal cells restore the senescence-clearance-regeneration sequence that is impaired in chronic muscle inflammation
    Authors: TS Chikenji, Y Saito, N Konari, M Nakano, Y Mizue, M Otani, M Fujimiya
    EBioMedicine, 2019;0(0):.  2019
  21. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019;45(0):511-518.  2019
  22. The Secretomes of Painful Versus Nonpainful Human Schwannomatosis Tumor Cells Differentially Influence Sensory Neuron Gene Expression and Sensitivity
    Authors: KL Ostrow, KJ Donaldson, MJ Caterina, A Belzberg, A Hoke
    Sci Rep, 2019;9(1):13098.  2019
  23. The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues
    Authors: Y Wada, T Ikemoto, Y Morine, S Imura, Y Saito, S Yamada, M Shimada
    Sci Rep, 2019;9(1):13204.  2019
  24. Astrogliosis Releases Pro-Oncogenic Chitinase 3-Like 1 Causing MAPK Signaling in Glioblastoma
    Authors: J Wurm, SP Behringer, VM Ravi, K Joseph, N Neidert, JP Maier, R Doria-Medi, M Follo, D Delev, D Pfeifer, J Beck, R Sankowski, O Schnell, DH Heiland
    Cancers (Basel), 2019;11(10):.  2019
  25. Radiotherapy-Induced Changes in the Systemic Immune and Inflammation Parameters of Head and Neck Cancer Patients
    Authors: K Balázs, E Kis, C Badie, EN Bogdándi, S Candéias, LC Garcia, I Dominczyk, B Frey, U Gaipl, Z Jurányi, ZS Kocsis, EA Rutten, G Sáfrány, P Widlak, K Lumniczky
    Cancers (Basel), 2019;11(9):.  2019
  26. CCR2 signaling in breast carcinoma cells promotes tumor growth and invasion by promoting CCL2 and suppressing CD154 effects on the angiogenic and immune microenvironments
    Authors: G Brummer, W Fang, C Smart, B Zinda, N Alissa, C Berkland, D Miller, N Cheng
    Oncogene, 2019;0(0):.  2019
  27. Obesity in acute alcoholic hepatitis increases morbidity and mortality
    Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
    EBioMedicine, 2019;45(0):511-518.  2019
  28. Interleukin-6 mediates resistance to PI3K-pathway-targeted therapy in lymphoma
    Authors: JH Kim, WS Kim, C Park
    BMC Cancer, 2019;19(1):936.  2019
  29. PRMT6 promotes lung tumor progression via the alternate activation of tumor-associated macrophages
    Authors: S Avasarala, PY Wu, SQ Khan, S Yanlin, M Van Scoyk, J Bao, A Di Lorenzo, O David, MT Bedford, V Gupta, RA Winn, RK Bikkavilli
    Mol. Cancer Res., 2019;0(0):.  2019
  30. Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis
    Authors: E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner
    Cell Death Dis, 2019;10(10):729.  2019
  31. Extracellular Membrane Vesicles from Lactobacilli Dampen IFN-gamma Responses in a Monocyte-Dependent Manner
    Authors: M Mata Forsb, S Björkander, Y Pang, L Lundqvist, M Ndi, M Ott, IB Escribá, MC Jaeger, S Roos, E Sverremark
    Sci Rep, 2019;9(1):17109.  2019
  32. Inflammation-Related Patterns in the Clinical Staging and Severity Assessment of Chronic Kidney Disease
    Authors: S Mihai, E Codrici, ID Popescu, AM Enciu, E Rusu, D Zilisteanu, LG Necula, G Anton, C Tanase
    Dis. Markers, 2019;2019(0):1814304.  2019
  33. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
    Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
    Int J Mol Sci, 2019;20(3):.  2019
  34. Physiologic expansion of human heart-derived cells enhances therapeutic repair of injured myocardium
    Authors: S Mount, P Kanda, S Parent, S Khan, C Michie, L Davila, V Chan, RA Davies, H Haddad, D Courtman, DJ Stewart, DR Davis
    Stem Cell Res Ther, 2019;10(1):316.  2019
  35. Mesenchymal stem cell senescence alleviates their intrinsic and seno-suppressive paracrine properties contributing to osteoarthritis development
    Authors: O Malaise, Y Tachikart, M Constantin, M Mumme, R Ferreira-L, S Noack, C Krettek, D Noël, J Wang, C Jorgensen, JM Brondello
    Aging (Albany NY), 2019;11(20):9128-9146.  2019
  36. Ectoine-Containing Inhalation Solution versus Saline Inhalation Solution in the Treatment of Acute Bronchitis and Acute Respiratory Infections: A Prospective, Controlled, Observational Study
    Authors: BH Tran, VA Dao, A Bilstein, K Unfried, K Shah-Hosse, R Mösges
    Biomed Res Int, 2019;2019(0):7945091.  2019
  37. Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis
    Authors: W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, X Wu, Y Wu, H Shen
    Cell Death Dis, 2019;10(3):188.  2019
  38. Genetic and pharmacological validation of TAK1 inhibition in macrophages as a therapeutic strategy to effectively inhibit TNF secretion
    Authors: SA Scarneo, A Mansourati, LS Eibschutz, J Totzke, JR Roques, D Loiselle, D Carlson, P Hughes, TAJ Haystead
    Sci Rep, 2018;8(1):17058.  2018
  39. ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
    Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
    Sci Rep, 2018;8(1):1431.  2018
  40. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.  2018
  41. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018;24(9):2300-2311.  2018
  42. Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
    Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
    Cell Death Dis, 2018;9(2):154.  2018
  43. Gle1 mediates stress granule-dependent survival during chemotoxic stress
    Authors: L Glass, SR Wente
    Adv Biol Regul, 2018;0(0):.  2018
  44. Unique and shared inflammatory profiles of human brain endothelia and pericytes
    Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
    J Neuroinflammation, 2018;15(1):138.  2018
  45. Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
    Authors: JM Cha, EK Shin, JH Sung, GJ Moon, EH Kim, YH Cho, HD Park, H Bae, J Kim, OY Bang
    Sci Rep, 2018;8(1):1171.  2018
  46. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018;4(2):e1701729.  2018
  47. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
    Nature, 2018;0(0):.  2018
  48. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018;0(0):.  2018
  49. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018;0(0):.  2018
  50. CST3 and GDF15 ameliorate renal fibrosis by inhibiting fibroblast growth and activation
    Authors: YI Kim, HW Shin, YS Chun, JW Park
    Biochem. Biophys. Res. Commun., 2018;0(0):.  2018
  51. Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
    Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
    J. Cell. Mol. Med., 2018;0(0):.  2018
  52. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018;9(0):1025.  2018
  53. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
    Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
    Int J Med Sci, 2018;15(12):1365-1372.  2018
  54. A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
    Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
    Cell Rep, 2018;25(13):3706-3720.e8.  2018
  55. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018;9(5):506.  2018
  56. Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts
    Authors: T Kifune, H Ito, M Ishiyama, S Iwasa, H Takei, T Hasegawa, M Asano, T Shirakawa
    J Oral Sci, 2018;60(4):519-525.  2018
  57. Ca2+ Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
    Authors: K Eubler, C Herrmann, A Tiefenbach, FM Köhn, JU Schwarzer, L Kunz, A Mayerhofer
    Int J Mol Sci, 2018;19(9):.  2018
  58. Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
    Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
    Am. J. Pathol., 2018;188(4):950-966.  2018
  59. Withaferin A Associated Differential Regulation of Inflammatory Cytokines
    Authors: S Dubey, H Yoon, MS Cohen, P Nagarkatti, M Nagarkatti, D Karan
    Front Immunol, 2018;9(0):195.  2018
  60. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
    Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
    Nat Commun, 2018;9(1):3996.  2018
  61. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
    Authors: MD Wood, J Mukherjee, RO Pieper
    Sci Rep, 2018;8(1):5805.  2018
  62. Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
    Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
    J. Immunol., 2017;0(0):.  2017
  63. An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
    Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
    Oncotarget, 2017;8(15):24869-24881.  2017
  64. Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis
    Authors: K Jin, NB Pandey, AS Popel
    Oncotarget, 2017;0(0):.  2017
  65. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.  2017
  66. Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
    Authors: AL Strong, DT Pei, CG Hurst, JM Gimble, ME Burow, BA Bunnell
    Stem Cells Int, 2017;2017(0):9216502.  2017
  67. Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
    Authors: JB Moore, J Zhao, AG Fischer, MCL Keith, D Hagan, M Wysoczynsk, R Bolli
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    Authors: Etsuko Matsumura
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Do I need to use the protease inhibitors (e.g. aprotinin, Ipegal) if I only plan to use cell supernatants?

    • The proteinase inhibitors are recommended for cell and tissue lysates only.  You do not need them for cell culture supernates.

  3. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Human XL Cytokine Array Kit

Average Rating: 4.6 (Based on 14 Reviews)

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Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 04/29/2021
Application:

Used 1 ml of pooled basal media from air-liquid interface cultures of primary epithelial cells

Protocol very easy to follow, but data analysis can be tricky. Would benefit massively from coming with its own analysis tool / ImageJ plugin.

Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 10/20/2020
Application:
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 05/22/2020
Application:

220 uL of rat plasma for overnight incubation with arrays at 4c.

Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 02/18/2020
Application:

Human endothelial cells treated +/- FAK kinase inhibitors +/- doxorubicin

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 01/14/2020
Application:

Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111

Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 07/01/2019
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/07/2019
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/06/2019
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 02/07/2019
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 01/03/2019
Application:
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 12/26/2018
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 12/12/2018
Application:

THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.

Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/14/2018
Application:
Serum Proteome Profiler Human XL Cytokine Array Kit ARY022B
Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 04/01/2018
Application: