Proteome Profiler Human XL Cytokine Array Kit
Proteome Profiler Human XL Cytokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.
- Detects 105 human cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Array
The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human XL Cytokine Array
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
|Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.|
|Apolipoprotein A1||IL-1 beta/IL-1F2||CXCL9/MIG|
|BAFF/BLyS/TNFSF13B||IL-1ra/IL-1F3||CCL3/CCL4 MIP-1 alpha/beta|
|CD40 Ligand/TNFSF5||IL-6||Osteopontin (OPN)|
|Complement Component C5/C5a||IL-10||PDGF-AB/BB|
|Complement Factor D||IL-11||Pentraxin 3/TSF-14|
|C-Reactive Protein/CRP||IL-12 p70||CXCL4/PF4|
|Fas Ligand||IL-24||ST2/IL1 R4|
|CXCL1/GRO alpha||Kallikrein 3/PSA||uPAR|
|Growth Hormone (GH)||Leptin||VCAM-1|
|ICAM-1/CD54||Lipocalin-2/NGAL||Vitamin D BP|
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
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Figure 1A. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours.
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Figure 1B. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours.
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Figure 1C. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours.
Preparation and Storage
Citations for Proteome Profiler Human XL Cytokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
J. Cell. Mol. Med., 2018;0(0):. 2018
Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
Cell Death Dis, 2018;9(5):506. 2018
Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
Cancer Med, 2018;0(0):. 2018
A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
J Oral Sci, 2018;0(0):. 2018
Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
Front Physiol, 2018;9(0):1025. 2018
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
Nat Commun, 2018;9(1):3267. 2018
Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
Cell Rep, 2018;24(9):2300-2311. 2018
Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
J Am Heart Assoc, 2017;6(5):. 2017
Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
J. Immunol., 2017;0(0):. 2017
Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
Sci Rep, 2017;7(1):10608. 2017
Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
Sci Rep, 2016;6(0):29588. 2016
Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
PLoS Pathog, 2015;11(12):e1005319. 2015
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