Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Proteome Profiler Human XL Cytokine Array Kit
R&D Systems | Catalog # ARY022B
Key Product Details
Species
Product Summary for Proteome Profiler Human XL Cytokine Array Kit
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.
Key Benefits
- Detects 105 human cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Array
The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
|---|---|---|
| Adiponectin/Acrp30 | IFN-gamma | CCL2/MCP-1 |
| Angiogenin | IGFBP-2 | CCL7/MCP-3 |
| Angiopoietin-1 | IGFBP-3 | M-CSF |
| Angiopoietin-2 | IL-1 alpha/IL-1F1 | MIF |
| Apolipoprotein A1 | IL-1 beta/IL-1F2 | CXCL9/MIG |
| BAFF/BLyS/TNFSF13B | IL-1ra/IL-1F3 | CCL3/CCL4 MIP-1 alpha/beta |
| BDNF | IL-2 | CCL20/MIP-3 alpha |
| CD14 | IL-3 | CCL19/MIP-3 beta |
| CD30 | IL-4 | MMP-9 |
| CD31/PECAM-1 | IL-5 | Myeloperoxidase |
| CD40 Ligand/TNFSF5 | IL-6 | Osteopontin (OPN) |
| Chitinase 3-like | IL-8 | PDGF-AA |
| Complement Component C5/C5a | IL-10 | PDGF-AB/BB |
| Complement Factor D | IL-11 | Pentraxin 3/TSF-14 |
| C-Reactive Protein/CRP | IL-12 p70 | CXCL4/PF4 |
| Cripto-1 | IL-13 | RAGE |
| Cystatin C | IL-15 | CCL5/RANTES |
| Dkk-1 | IL-16 | RBP4 |
| DPPIV/CD26 | IL-17A | Relaxin-2 |
| EGF | IL-18 BPa | Resistin |
| CXCL5/ENA-78 | IL-19 | CXCL12/SDF-1 alpha |
| Endoglin/CD105 | IL-22 | Serpin E1/PAI-1 |
| EMMPRIN | IL-23 | SHBG |
| Fas Ligand | IL-24 | ST2/IL1 R4 |
| FGF basic | IL-27 | CCL17/TARC |
| KGF/FGF-7 | IL-31 | TFF3 |
| FGF-19 | IL-32 alpha/beta/gamma | TfR |
| Flt-3 Ligand | IL-33 | TGF-alpha |
| G-CSF | IL-34 | Thrombospondin-1 |
| GDF-15 | CXCL10/IP-10 | TIM-3 |
| GM-CSF | CXCL11/I-TAC | TNF-alpha |
| CXCL1/GRO alpha | Kallikrein 3/PSA | uPAR |
| Growth Hormone (GH) | Leptin | VCAM-1 |
| HGF | LIF | VEGF |
| ICAM-1/CD54 | Lipocalin-2/NGAL | Vitamin D BP |
Scientific Data Images for Proteome Profiler Human XL Cytokine Array Kit
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
Formulation, Preparation, and Storage
Shipping
Storage
Background: Multiplex Cytokine Assay Kits
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Human XL Cytokine Array Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Proteome Profiler Human XL Cytokine Array Kit
For research use only
Citations for Proteome Profiler Human XL Cytokine Array Kit
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Customer Reviews for Proteome Profiler Human XL Cytokine Array Kit (22)
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Customer Images
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Verified Customer | Posted 11/29/2024Cell culture supernatant from organoids culture. Exposure of 2 minutes
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Verified Customer | Posted 11/29/2024350 uL of cell culture medium
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Verified Customer | Posted 12/11/2023LI-COR
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Verified Customer | Posted 07/11/2022
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Verified Customer | Posted 05/13/2022Product was easy to use and quick. In house analysis software would improve the process. Array has not been tested if the specific antibodies differentiate between precursor and mature proteins or bound vs unbound proteins. Testing this would increase applicability. Image provided is an example of 100 ul of human serum.
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Verified Customer | Posted 12/02/2021
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Verified Customer | Posted 10/21/2021
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Verified Customer | Posted 09/07/2021
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Verified Customer | Posted 04/29/2021Used 1 ml of pooled basal media from air-liquid interface cultures of primary epithelial cells Protocol very easy to follow, but data analysis can be tricky. Would benefit massively from coming with its own analysis tool / ImageJ plugin.
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Verified Customer | Posted 10/20/2020
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Verified Customer | Posted 05/22/2020220 uL of rat plasma for overnight incubation with arrays at 4c.
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Verified Customer | Posted 02/18/2020Human endothelial cells treated +/- FAK kinase inhibitors +/- doxorubicin
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Verified Customer | Posted 01/14/2020Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111
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Verified Customer | Posted 07/01/2019
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Verified Customer | Posted 06/07/2019
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Verified Customer | Posted 06/06/2019
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Verified Customer | Posted 02/07/2019
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Verified Customer | Posted 01/03/2019
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Verified Customer | Posted 12/26/2018
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Verified Customer | Posted 12/12/2018THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.
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Verified Customer | Posted 09/14/2018
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Verified Customer | Posted 04/01/2018
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FAQs for Proteome Profiler Human XL Cytokine Array Kit
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Q: Does the MMP-9 antibody detect TIMP and MMP-9 complex?
A: Proteome Profiler Arrays are semi-quantitative screening tools and are broadly validated for multiplexing without extensive characterization. This array is expected to detect total MMP-9, which includes pro-, active, and TIMP complexed MMP-9. R&D Systems ELISA kits are available for further quantification of MMP-9.
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Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.
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Q: Does the MMP-9 antibody detect TIMP and MMP-9 complex?
A: Proteome Profiler Arrays are semi-quantitative screening tools and are broadly validated for multiplexing without extensive characterization. This array is expected to detect total MMP-9, which includes pro-, active, and TIMP complexed MMP-9. R&D Systems ELISA kits are available for further quantification of MMP-9.
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Q: Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?
A: The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment.