Proteome Profiler Human XL Cytokine Array Kit

Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY022B
Product Details
Citations (31)
FAQs
Reviews (2)

Proteome Profiler Human XL Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 105 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma CCL2/MCP-1
Angiogenin IGFBP-2 CCL7/MCP-3
Angiopoietin-1 IGFBP-3 M-CSF
Angiopoietin-2 IL-1 alpha/IL-1F1 MIF
Apolipoprotein A1 IL-1 beta/IL-1F2 CXCL9/MIG
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CCL3/CCL4 MIP-1 alpha/beta
BDNF IL-2 CCL20/MIP-3 alpha
CD14 IL-3 CCL19/MIP-3 beta
CD30 IL-4 MMP-9
CD31/PECAM-1 IL-5 Myeloperoxidase
CD40 Ligand/TNFSF5 IL-6 Osteopontin (OPN)
Chitinase 3-like IL-8 PDGF-AA
Complement Component C5/C5a IL-10 PDGF-AB/BB
Complement Factor D IL-11 Pentraxin 3/TSF-14
C-Reactive Protein/CRP IL-12 p70 CXCL4/PF4
Cripto-1 IL-13 RAGE
Cystatin C IL-15 CCL5/RANTES
Dkk-1 IL-16 RBP4
DPPIV/CD26 IL-17A Relaxin-2
EGF IL-18 BPa Resistin
CXCL5/ENA-78 IL-19 CXCL12/SDF-1 alpha
Endoglin/CD105 IL-22 Serpin E1/PAI-1
EMMPRIN IL-23 SHBG
Fas Ligand IL-24 ST2/IL1 R4
FGF basic IL-27 CCL17/TARC
KGF/FGF-7 IL-31 TFF3
FGF-19 IL-32 alpha/beta/gamma TfR
Flt-3 Ligand IL-33 TGF-alpha
G-CSF IL-34 Thrombospondin-1
GDF-15 CXCL10/IP-10 TIM-1
GM-CSF CXCL11/I-TAC TNF-alpha
CXCL1/GRO alpha Kallikrein 3/PSA uPAR
Growth Hormone (GH) Leptin VCAM-1
HGF LIF VEGF
ICAM-1/CD54 Lipocalin-2/NGAL Vitamin D BP

 

Data Examples

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

ARY022B Figure 1A
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Figure 1A. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours.

ARY022B Figure 1B
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Figure 1B. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours.

ARY022B Figure 1C
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Figure 1C. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
    Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
    Nat Commun, 2018;9(1):3996.  2018
  2. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018;24(9):2300-2311.  2018
  3. Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
    Authors: MD Wood, J Mukherjee, RO Pieper
    Sci Rep, 2018;8(1):5805.  2018
  4. ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
    Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
    Sci Rep, 2018;8(1):1431.  2018
  5. IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
    Nature, 2018;0(0):.  2018
  6. Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
    Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
    Sci Adv, 2018;4(2):e1701729.  2018
  7. Unique and shared inflammatory profiles of human brain endothelia and pericytes
    Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
    J Neuroinflammation, 2018;15(1):138.  2018
  8. Gle1 mediates stress granule-dependent survival during chemotoxic stress
    Authors: L Glass, SR Wente
    Adv Biol Regul, 2018;0(0):.  2018
  9. Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
    Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
    Cell Death Dis, 2018;9(2):154.  2018
  10. Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
    Authors: JM Cha, EK Shin, JH Sung, GJ Moon, EH Kim, YH Cho, HD Park, H Bae, J Kim, OY Bang
    Sci Rep, 2018;8(1):1171.  2018
  11. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.  2018
  12. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018;0(0):.  2018
  13. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018;0(0):.  2018
  14. Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
    Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
    J. Cell. Mol. Med., 2018;0(0):.  2018
  15. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018;9(0):1025.  2018
  16. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018;9(5):506.  2018
  17. Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
    Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
    J. Immunol., 2017;0(0):.  2017
  18. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.  2017
  19. Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
    Authors: JB Moore, J Zhao, AG Fischer, MCL Keith, D Hagan, M Wysoczynsk, R Bolli
    J Am Heart Assoc, 2017;6(7):.  2017
  20. Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
    Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
    Sci Rep, 2017;7(1):10608.  2017
  21. An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
    Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
    Oncotarget, 2017;8(15):24869-24881.  2017
  22. Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis
    Authors: K Jin, NB Pandey, AS Popel
    Oncotarget, 2017;0(0):.  2017
  23. Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
    Authors: AL Strong, DT Pei, CG Hurst, JM Gimble, ME Burow, BA Bunnell
    Stem Cells Int, 2017;2017(0):9216502.  2017
  24. Protective Effect of Let-7 miRNA Family in Regulating Inflammation in Diabetes-Associated Atherosclerosis
    Authors: E Brennan, B Wang, A McClelland, M Mohan, M Marai, O Beuscart, S Derouiche, S Gray, R Pickering, C Tikellis, M de Gaetano, M Barry, O Belton, ST Ali-Shah, P Guiry, KA Jandeleit-, ME Cooper, C Godson, P Kantharidi
    Diabetes, 2017;0(0):.  2017
  25. Pretreatment of IL-1? enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells
    Authors: Etsuko Matsumura
    Cytotherapy, 2016;0(0):.  2016
  26. Identification of Pathways in Liver Repair Potentially Targeted by Secretory Proteins from Human Mesenchymal Stem Cells
    Int J Mol Sci, 2016;17(7):.  2016
  27. Diesel Exhaust Particles Upregulate Interleukins IL-6 and IL-8 in Nasal Fibroblasts
    Authors: Jin Ah Kim
    PLoS ONE, 2016;11(6):e0157058.  2016
  28. Transcriptional upregulation of c-MET is associated with invasion and tumor budding in colorectal cancer
    Oncotarget, 2016;0(0):.  2016
  29. Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer
    Nature, 2016;533(7604):493-8.  2016
  30. Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
    Sci Rep, 2016;6(0):29588.  2016
  31. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
    Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
    PLoS Pathog, 2015;11(12):e1005319.  2015

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