Proteome Profiler Human XL Cytokine Array Kit

Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
Catalog # Availability Size / Price Qty

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The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates.
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Citations (125)
Reviews (19)

Proteome Profiler Human XL Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 105 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.


Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.


Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma CCL2/MCP-1
Angiogenin IGFBP-2 CCL7/MCP-3
Angiopoietin-1 IGFBP-3 M-CSF
Angiopoietin-2 IL-1 alpha/IL-1F1 MIF
Apolipoprotein A1 IL-1 beta/IL-1F2 CXCL9/MIG
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CCL3/CCL4 MIP-1 alpha/beta
BDNF IL-2 CCL20/MIP-3 alpha
CD14 IL-3 CCL19/MIP-3 beta
CD30 IL-4 MMP-9
CD31/PECAM-1 IL-5 Myeloperoxidase
CD40 Ligand/TNFSF5 IL-6 Osteopontin (OPN)
Chitinase 3-like IL-8 PDGF-AA
Complement Component C5/C5a IL-10 PDGF-AB/BB
Complement Factor D IL-11 Pentraxin 3/TSF-14
C-Reactive Protein/CRP IL-12 p70 CXCL4/PF4
Cripto-1 IL-13 RAGE
Cystatin C IL-15 CCL5/RANTES
Dkk-1 IL-16 RBP4
DPPIV/CD26 IL-17A Relaxin-2
EGF IL-18 BPa Resistin
CXCL5/ENA-78 IL-19 CXCL12/SDF-1 alpha
Endoglin/CD105 IL-22 Serpin E1/PAI-1
Fas Ligand IL-24 ST2/IL1 R4
FGF basic IL-27 CCL17/TARC
FGF-19 IL-32 alpha/beta/gamma TfR
Flt-3 Ligand IL-33 TGF-alpha
G-CSF IL-34 Thrombospondin-1
GDF-15 CXCL10/IP-10 TIM-1
CXCL1/GRO alpha Kallikrein 3/PSA uPAR
Growth Hormone (GH) Leptin VCAM-1
ICAM-1/CD54 Lipocalin-2/NGAL Vitamin D BP


Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Datasheets

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Data Example

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

125 Citations: Showing 1 - 10
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  1. LCN2 is a new diagnostic biomarker and potential therapeutic target in idiopathic short stature
    Authors: X Liu, J Zhang, J Yuan, R Ding, T Liu, J Jia
    Journal of Cellular and Molecular Medicine, 2022;0(0):.  2022
  2. Chemerin Overexpression in the Liver Protects against Inflammation in Experimental Non-Alcoholic Steatohepatitis
    Authors: R Pohl, S Feder, EM Haberl, L Rein-Fisch, TS Weiss, M Spirk, A Bruckmann, N McMullen, CJ Sinal, C Buechler
    Biomedicines, 2022;10(1):.  2022
  3. Isolation of Platelet-Derived Exosomes from Human Platelet-Rich Plasma: Biochemical and Morphological Characterization
    Authors: M Saumell-Es, D Delgado, G García Del, M Beitia, J Sallés, I González-B, P Sánchez, M López de J, S Barrondo, M Sánchez
    International Journal of Molecular Sciences, 2022;23(5):.  2022
  4. Cancer-associated fibroblast-induced lncRNA UPK1A-AS1 confers platinum resistance in pancreatic cancer via efficient double-strand break repair
    Authors: X Zhang, S Zheng, C Hu, G Li, H Lin, R Xia, Y Ye, R He, Z Li, Q Lin, R Chen, Q Zhou
    Oncogene, 2022;0(0):.  2022
  5. Ibuprofen in Therapeutic Concentrations Affects the Secretion of Human Bone Marrow Mesenchymal Stromal Cells, but Not Their Proliferative and Migratory Capacity
    Authors: A Kulesza, K Zielniok, J Hawryluk, L Paczek, A Burdzinska
    Biomolecules, 2022;12(2):.  2022
  6. Comprehensive Molecular Landscape of Cetuximab Resistance in Head and Neck Cancer Cell Lines
    Authors: INF Gomes, RJ da Silva-O, LS da Silva, O Martinho, AF Evangelist, A van Helvoo, LF Leal, VAO Silva, SP Dos Santos, FC Nascimento, A Lopes Carv, RM Reis
    Cells, 2022;11(1):.  2022
  7. Preferential stimulation of melanocytes by M2 macrophages to produce melanin through vascular endothelial growth factor
    Authors: H Han, Y Kim, H Mo, SH Choi, K Lee, YA Rim, JH Ju
    Scientific Reports, 2022;12(1):6416.  2022
  8. Analysis of gingival crevicular fluid biomarkers in patients with metabolic syndrome
    Authors: L Nibali, A Stephen, E Hagi-Pavli, R Allaker, AD Pino, V Terranova, M Pisano, SD Marca, V Ferrara, R Scicali, M Giordano, F Purrello, N Donos, L Malatino
    Journal of dentistry, 2022;118(0):104065.  2022
  9. Na�ve Human Macrophages Are Refractory to SARS-CoV-2 Infection and Exhibit a Modest Inflammatory Response Early in Infection
    Authors: Z Zhang, R Penn, WS Barclay, ES Giotis
    Viruses, 2022;14(2):.  2022
  10. ERalpha36-High Cancer-Associated Fibroblasts as an Unfavorable Factor in Triple-Negative Breast Cancer
    Authors: A Nagel, M Popeda, A Muchlinska, R Sadej, J Szade, J Zielinski, J Skokowski, M Niemira, A Kretowski, A Markiewicz, AJ Zaczek
    Cancers, 2022;14(8):.  2022
  11. Efficacy of chitinase-3-like protein 1 as an in�vivo bone formation predictable marker of maxillary/mandibular bone marrow stromal cells
    Authors: N Komabashir, F Suehiro, M Ishii, M Nishimura
    Regenerative Therapy, 2021;18(0):38-50.  2021
  12. Suprabasin-derived bioactive peptides identified by plasma peptidomics
    Authors: T Taguchi, Y Kodera, K Oba, T Saito, Y Nakagawa, Y Kawashima, M Shichiri
    Scientific Reports, 2021;11(1):1047.  2021
  13. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
    Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
    Leukemia, 2021;0(0):.  2021
  14. Cancer testis antigen PRAME: An anti-cancer target with immunomodulatory potential
    Authors: A Naik, R Thomas, G Al-Khadair, R Bacha, W Hendrickx, J Decock
    Journal of Cellular and Molecular Medicine, 2021;0(0):.  2021
  15. Electrophysiological engineering of heart-derived cells with calcium-dependent potassium channels improves cell therapy efficacy for cardioprotection
    Authors: P Vigneault, S Parent, P Kanda, C Michie, DR Davis, S Nattel
    Nature Communications, 2021;12(1):4963.  2021
  16. CD276 is an important player in macrophage recruitment into the tumor and an upstream regulator for PAI-1
    Authors: S Durlanik, K Fundel-Cle, C Viollet, HJ Huber, M Lenter, K Kitt, S Pflanz
    Scientific Reports, 2021;11(1):14849.  2021
  17. Altered Electrical, Biomolecular, and Immunologic Phenotypes in a Novel Patient-Derived Stem Cell Model of Desmoglein-2 Mutant ARVC
    Authors: RN Hawthorne, A Blazeski, J Lowenthal, S Kannan, R Teuben, D DiSilvestr, J Morrissett, JE Saffitz, KR Boheler, CA James, SP Chelko, G Tomaselli, L Tung
    Journal of Clinical Medicine, 2021;10(14):.  2021
  18. Influence of serum inflammatory cytokines on cytochrome P450 drug metabolising activity during breast cancer chemotherapy: a patient feasibility study
    Authors: RLI Crake, MR Strother, E Phillips, MP Doogue, M Zhang, CMA Frampton, BA Robinson, MJ Currie
    Scientific Reports, 2021;11(1):5648.  2021
  19. Adhesion strength and contractility enable metastatic cells to become adurotactic
    Authors: B Yeoman, G Shatkin, P Beri, A Banisadr, P Katira, AJ Engler
    Cell Reports, 2021;34(10):108816.  2021
  20. Tear-Derived Exosome Proteins Are Increased in Patients with Thyroid Eye Disease
    Authors: JS Han, SE Kim, JQ Jin, NR Park, JY Lee, HL Kim, SB Lee, SW Yang, DJ Lim
    International Journal of Molecular Sciences, 2021;22(3):.  2021
  21. Combination of Epithelial Growth Factor Receptor Blockers and CDK4/6 Inhibitor for Nasopharyngeal Carcinoma Treatment
    Authors: HP Li, CY Huang, KW Lui, YK Chao, CN Yeh, LY Lee, Y Huang, TL Lin, YC Kuo, MY Huang, YR Lai, YM Yeh, HC Fan, AC Lin, JC Hsieh, KP Chang, CY Lin, HM Wang, YS Chang, CL Hsu
    Cancers, 2021;13(12):.  2021
  22. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021;12(1):3543.  2021
  23. Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age
    Authors: D Delgado, AM Bilbao, M Beitia, A Garate, P Sánchez, I González-B, A Isasti, M López De J, J Zuazo-Ibar, A Montilla, M Domercq, E Capetillo-, G García Del, J Sallés, C Matute, M Sánchez
    International Journal of Molecular Sciences, 2021;22(4):.  2021
  24. Inhibition of tumor progression and M2 microglial polarization by extracellular vesicle-mediated microRNA-124 in a 3D microfluidic glioblastoma microenvironment
    Authors: S Hong, JY You, K Paek, J Park, SJ Kang, EH Han, N Choi, S Chung, WJ Rhee, JA Kim
    Theranostics, 2021;11(19):9687-9704.  2021
  25. CD109-GP130 interaction drives glioblastoma stem cell plasticity and chemoresistance through STAT3 activity
    Authors: P Filppu, JT Ramanathan, KJ Granberg, E Gucciardo, H Haapasalo, K Lehti, M Nykter, V Le Joncour, P Laakkonen
    JCI Insight, 2021;6(9):.  2021
  26. Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression
    Authors: M Shimizu, YI Koma, H Sakamoto, S Tsukamoto, Y Kitamura, S Urakami, K Tanigawa, T Kodama, N Higashino, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki
    Cancers, 2021;13(18):.  2021
  27. Reduction of Oxidative Stress in Peripheral Blood Mononuclear Cells Attenuates the Inflammatory Response of Fibroblast-like Synoviocytes in Rheumatoid Arthritis
    Authors: HR Lee, SJ Yoo, J Kim, CK Park, SW Kang
    International Journal of Molecular Sciences, 2021;22(22):.  2021
  28. Gelatin methacrylate hydrogels culture model for glioblastoma cells enriches for mesenchymal-like state and models interactions with immune cells
    Authors: N Shah, PM Hallur, RA Ganesh, P Sonpatki, D Naik, KP Chandracha, RB Puchalski, A Chaubey
    Scientific Reports, 2021;11(1):17727.  2021
  29. Lnc-DC promotes estrogen independent growth and tamoxifen resistance in breast cancer
    Authors: WX Peng, P Koirala, H Zhou, J Jiang, Z Zhang, L Yang, YY Mo
    Cell Death & Disease, 2021;12(11):1000.  2021
  30. Transcryptomic Analysis of Human Brain-Microvascular Endothelial Response to -Pericytes: Cell Orientation Defines Barrier Function
    Authors: L Kurmann, M Okoniewski, OO Ogunshola, B Leeners, B Imthurn, RK Dubey
    Cells, 2021;10(4):.  2021
  31. GSTM1 Modulates Expression of Endothelial Adhesion Molecules in Uremic Milieu
    Authors: D Jerotic, S Suvakov, M Matic, A Alqudah, DJ Grieve, M Pljesa-Erc, A Savic-Rado, T Damjanovic, N Dimkovic, L McClements, T Simic
    Oxidative Medicine and Cellular Longevity, 2021;2021(0):6678924.  2021
  32. Diphenylarsinic acid induced activation of MAP kinases, transcription factors, and oxidative stress-responsive factors and hypersecretion of cytokines in cultured normal human cerebellar astrocytes
    Authors: S Sasaki, T Negishi, T Tsuzuki, K Yukawa
    Neurotoxicology, 2021;88(0):196-207.  2021
  33. C-C Motif Chemokine Ligand-17 as a Novel Biomarker and Regulator of Epithelial Mesenchymal Transition in Renal Fibrogenesis
    Authors: YH Hsieh, WC Wang, TW Hung, CC Lee, JP Tsai
    Cells, 2021;10(12):.  2021
  34. Transcryptomic Analysis of Human Brain -Microvascular Endothelial Cell Driven Changes in -Vascular Pericytes
    Authors: L Kurmann, M Okoniewski, RK Dubey
    Cells, 2021;10(7):.  2021
  35. Dynamic prostate cancer transcriptome analysis delineates the trajectory to disease progression
    Authors: M Bolis, D Bossi, A Vallerga, V Ceserani, M Cavalli, D Impellizzi, L Di Rito, E Zoni, S Mosole, AR Elia, A Rinaldi, R Pereira Me, E D'Antonio, M Ferrari, F Stoffel, F Jermini, S Gillessen, L Bubendorf, P Schraml, A Calcinotto, E Corey, H Moch, M Spahn, G Thalmann, M Kruithof-d, MA Rubin, JP Theurillat
    Nature Communications, 2021;12(1):7033.  2021
  36. TAK1 regulates the tumor microenvironment through inflammatory, angiogenetic and apoptotic signaling cascades
    Authors: SA Scarneo, KW Yang, JR Roques, A Dai, LS Eibschutz, P Hughes, TAJ Haystead
    Oncotarget, 2020;11(21):1961-1970.  2020
  37. Human amniotic mesenchymal stromal cells promote bone regeneration via activating endogenous regeneration
    Authors: F Jiang, W Zhang, M Zhou, Z Zhou, M Shen, N Chen, X Jiang
    Theranostics, 2020;10(14):6216-6230.  2020
  38. Intrathecal Infusion of Autologous Adipose-Derived Regenerative Cells in Autoimmune Refractory Epilepsy: Evaluation of Safety and Efficacy
    Authors: E Szczepanik, H Mierzewska, D Antczak-Ma, A Figiel-Dab, I Terczynska, J Tryfon, N Krzesniak, BH Noszczyk, E Sawicka, K Domanska-J, A Sarnowska
    Stem Cells Int, 2020;2020(0):7104243.  2020
  39. Chemerin-156 is the Active Isoform in Human Hepatic Stellate Cells
    Authors: M Spirk, S Zimny, M Neumann, N McMullen, CJ Sinal, C Buechler
    Int J Mol Sci, 2020;21(20):.  2020
  40. Anti-CD3 Antibody Treatment Reduces Scar Formation in a Rat Model of Myocardial Infarction
    Authors: B Wernly, V Paar, A Aigner, PM Pilz, BK Podesser, M Förster, C Jung, JP Hofbauer, B Tockner, M Wimmer, T Kraus, LJ Motloch, M Hackl, UC Hoppe, A Kiss, M Lichtenaue
    Cells, 2020;9(2):.  2020
  41. Modulating Tumor-Associated Macrophage Polarization by Synthetic and Natural PPAR&gamma Ligands as a Potential Target in Breast Cancer
    Authors: G Gionfriddo, P Plastina, G Augimeri, S Catalano, C Giordano, I Barone, C Morelli, F Giordano, L Gelsomino, D Sisci, R Witkamp, S Andò, K van Norren, D Bonofiglio
    Cells, 2020;9(1):.  2020
  42. Permanent Pro-Tumorigenic Shift in Adipose Tissue-Derived Mesenchymal Stromal Cells Induced by Breast Malignancy
    Authors: J Plava, M Cihova, M Burikova, M Bohac, M Adamkov, S Drahosova, D Rusnakova, D Pindak, M Karaba, J Simo, M Mego, L Danisovic, L Kucerova, S Miklikova
    Cells, 2020;9(2):.  2020
  43. Nintedanib inhibits intrahepatic cholangiocarcinoma aggressiveness via suppression of cytokines extracted from activated cancer-associated fibroblasts
    Authors: T Yamanaka, N Harimoto, T Yokobori, R Muranushi, K Hoshino, K Hagiwara, D Gantumur, T Handa, N Ishii, M Tsukagoshi, T Igarashi, H Tanaka, A Watanabe, N Kubo, K Araki, K Shirabe
    Br. J. Cancer, 2020;0(0):.  2020
  44. SFMBT2-Mediated Infiltration of Preadipocytes and TAMs in Prostate Cancer
    Authors: J Gwak, H Jeong, K Lee, JY Shin, T Sim, J Na, J Kim, BG Ju
    Cancers, 2020;12(9):.  2020
  45. Zoledronic Acid Abrogates Restraint Stress-Induced Macrophage Infiltration, PDGF-AA Expression, and Ovarian Cancer Growth
    Authors: CB Colon-Eche, T Ortiz, L Maldonado, MJ Hidalgo-Va, J Pérez-Mora, AN Aquino-Ace, R Herrera-No, M Bonilla-Cl, EM Castro, GN Armaiz-Pen
    Cancers, 2020;12(9):.  2020
  46. Vadadustat, a HIF Prolyl Hydroxylase Inhibitor, Improves Immunomodulatory Properties of Human Mesenchymal Stromal Cells
    Authors: K Zielniok, A Burdzinska, B Kaleta, R Zagozdzon, L Paczek
    Cells, 2020;9(11):.  2020
  47. Excess of the NF-?B p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms
    Authors: Y Kravtsova-, G Goldhirsh, A Ivantsiv, O Ben Itzhak, YT Kwon, E Pikarsky, A Ciechanove
    Proc Natl Acad Sci U S A, 2020;0(0):.  2020
  48. Isolation and Characterization of Human Synovial Fluid-Derived Mesenchymal Stromal Cells from Popliteal Cyst
    Authors: F Li, J Chen, M Gong, Y Bi, C Hu, Y Zhang, M Li
    Stem Cells Int, 2020;2020(0):7416493.  2020
  49. Unique inflammatory signature in haemophilic arthropathy: miRNA changes due to interaction between blood and fibroblast-like synoviocytes
    Authors: S Mignot, N Cagnard, B Albaud, C Bally, J Siavellis, O Hermine, L Frenzel
    J Cell Mol Med, 2020;0(0):.  2020
  50. Tissue cytokine/chemokine profile in vulvar lichen sclerosus: An observational study on keratinocyte and fibroblast cultures
    Authors: M Corazza, L Oton-Gonza, V Scuderi, JC Rotondo, C Lanzillott, G Di Mauro, M Tognon, F Martini, A Borghi
    J Dermatol Sci, 2020;0(0):.  2020
  51. Acetylated K676 TGFBIp as a severity diagnostic blood biomarker for SARS-CoV-2 pneumonia
    Authors: HH Park, HN Kim, H Kim, Y Yoo, H Shin, EY Choi, JS Bae, W Lee
    Sci Adv, 2020;6(31):.  2020
  52. Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis
    Authors: G Ye, Z Xie, H Zeng, P Wang, J Li, G Zheng, S Wang, Q Cao, M Li, W Liu, S Cen, Z Li, Y Wu, Z Ye, H Shen
    Cell Death Dis, 2020;11(9):775.  2020
  53. Glycosaminoglycans as Tools to Decipher the Platelet Tumor Cell Interaction: A Focus on P-Selectin
    Authors: S Schwarz, LM Gockel, A Naggi, U Barash, M Gobec, G Bendas, M Schlesinge
    Molecules, 2020;25(5):.  2020
  54. Resveratrol as Inducer of Autophagy, Pro-Survival, and Anti-Inflammatory Stimuli in Cultured Human RPE Cells
    Authors: N Josifovska, R Albert, R Nagymihály, L Lytvynchuk, MC Moe, K Kaarnirant, ZJ Veréb, G Petrovski
    Int J Mol Sci, 2020;21(3):.  2020
  55. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages
    Authors: YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan
    Cancers, 2020;12(12):.  2020
  56. Serum-Derived Exosomal MicroRNA Profiles Can Predict Poor Survival Outcomes in Patients with Extranodal Natural Killer/T-Cell Lymphoma
    Authors: KJ Ryu, JY Lee, ME Choi, SE Yoon, J Cho, YH Ko, JH Shim, WS Kim, C Park, SJ Kim
    Cancers, 2020;12(12):.  2020
  57. Regulation of PD-L1 expression is a novel facet of cyclic-AMP-mediated immunosuppression
    Authors: B Sasi, P Ethiraj, J Myers, AP Lin, S Jiang, Z Qiu, KN Holder, RCT Aguiar
    Leukemia, 2020;0(0):.  2020
  58. Anti-EGFR Therapy Induces EGF Secretion by Cancer-Associated Fibroblasts to Confer Colorectal Cancer Chemoresistance
    Authors: CM Garvey, R Lau, A Sanchez, RX Sun, EJ Fong, ME Doche, O Chen, A Jusuf, HJ Lenz, B Larson, SM Mumenthale
    Cancers (Basel), 2020;12(6):.  2020
  59. Impact of Type 2 Diabetes Mellitus on Human Bone Marrow Stromal Cell Number and Phenotypic Characteristics
    Authors: FC Cassidy, C Shortiss, CG Murphy, SR Kearns, W Curtin, C De Buitléi, T O'Brien, CM Coleman
    Int J Mol Sci, 2020;21(7):.  2020
  60. Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies
    Authors: S Carluccio, D Martinelli, MEF Palamà, RC Pereira, R Benelli, A Guijarro, R Cancedda, C Gentili
    Cells, 2020;9(4):.  2020
  61. Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude
    Authors: A Gangwar, S Paul, Y Ahmad, K Bhargava
    Sci Rep, 2020;10(1):7899.  2020
  62. Reproducibility of GMP-compliant production of therapeutic stressed peripheral blood mononuclear cell-derived secretomes, a novel class of biological medicinal products
    Authors: M Laggner, A Gugerell, C Bachmann, H Hofbauer, V Vorstandle, M Seibold, G Gouya Lech, A Peterbauer, S Madlener, S Demyanets, D Sorgenfrey, T Ostler, M Erb, M Mildner, HJ Ankersmit
    Stem Cell Res Ther, 2020;11(1):9.  2020
  63. Mesenchymal stem cell senescence alleviates their intrinsic and seno-suppressive paracrine properties contributing to osteoarthritis development
    Authors: O Malaise, Y Tachikart, M Constantin, M Mumme, R Ferreira-L, S Noack, C Krettek, D Noël, J Wang, C Jorgensen, JM Brondello
    Aging (Albany NY), 2019;11(20):9128-9146.  2019
  64. The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues
    Authors: Y Wada, T Ikemoto, Y Morine, S Imura, Y Saito, S Yamada, M Shimada
    Sci Rep, 2019;9(1):13204.  2019
  65. Paradoxical effects of JZL184, an inhibitor of monoacylglycerol lipase, on bone remodelling in healthy and cancer-bearing mice
    Authors: S Marino, D de Ridder, RT Bishop, N Renema, M Ponzetti, A Sophocleou, M Capulli, A Aljeffery, G Carrasco, MD Gens, A Khogeer, SH Ralston, J Gertsch, F Lamoureux, D Heymann, N Rucci, AI Idris
    EBioMedicine, 2019;44(0):452-466.  2019
  66. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
    Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
    Int J Mol Sci, 2019;20(3):.  2019
  67. Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis
    Authors: W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, X Wu, Y Wu, H Shen
    Cell Death Dis, 2019;10(3):188.  2019
  68. Ectoine-Containing Inhalation Solution versus Saline Inhalation Solution in the Treatment of Acute Bronchitis and Acute Respiratory Infections: A Prospective, Controlled, Observational Study
    Authors: BH Tran, VA Dao, A Bilstein, K Unfried, K Shah-Hosse, R Mösges
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    Adv Biol Regul, 2018;0(0):.  2018
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  92. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
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  93. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
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    Nat Commun, 2018;9(1):3267.  2018
  94. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018;9(0):1025.  2018
  95. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018;0(0):.  2018
  96. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018;0(0):.  2018
  97. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018;9(5):506.  2018
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    Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
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  99. USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
    Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
    Nat Commun, 2018;9(1):3996.  2018
  100. Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
    Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
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    J Oral Sci, 2018;60(4):519-525.  2018
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  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

  2. Could you tell us whether this MMP-9 antibody can detect TIMP and MMP-9 complex?

    • We haven't performed a large amount of specificity testing per each spot in the arrays because these are qualitative screening tools. However, we would recommend treating the MMP-9 spot as a "Total MMP-9" detecting both free MMP-9 and MMP-9 in complex. If the MMP-9 spot turns out to be of high interest for the customer's study, they will want to examine this target in more detail with quantitative tools such as ELISA or Luminex.

View all Proteome Profiler Antibody Array FAQs

Reviews for Proteome Profiler Human XL Cytokine Array Kit

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Proteome Profiler Human XL Cytokine Array Kit
By Dong Hu on 07/11/2022

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 05/13/2022

Product was easy to use and quick. In house analysis software would improve the process. Array has not been tested if the specific antibodies differentiate between precursor and mature proteins or bound vs unbound proteins. Testing this would increase applicability. Image provided is an example of 100 ul of human serum.

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 12/02/2021

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 10/21/2021

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/07/2021

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 04/29/2021

Used 1 ml of pooled basal media from air-liquid interface cultures of primary epithelial cells

Protocol very easy to follow, but data analysis can be tricky. Would benefit massively from coming with its own analysis tool / ImageJ plugin.

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 10/20/2020

Proteome Profiler Human XL Cytokine Array Kit
By Dhanush Haspula on 05/22/2020

220 uL of rat plasma for overnight incubation with arrays at 4c.

Proteome Profiler Human XL Cytokine Array Kit
By Louise Reynolds on 02/18/2020

Human endothelial cells treated +/- FAK kinase inhibitors +/- doxorubicin

Proteome Profiler Human XL Cytokine Array Kit
By Lucia Lisi on 01/14/2020

Chiavari M, Ciotti GMP, Navarra P, Lisi L. Pro-Inflammatory Activation of A New Immortalized Human Microglia Cell Line. Brain Sci. 2019;9(5):111. Published 2019 May 15. doi:10.3390/brainsci9050111

Proteome Profiler Human XL Cytokine Array Kit
By Fadi Saadeh on 07/01/2019

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/07/2019

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 06/06/2019

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 02/07/2019

Proteome Profiler Human XL Cytokine Array Kit
By Ivan Luzardo on 01/03/2019

Proteome Profiler Human XL Cytokine Array Kit
By Samantha Scarneo on 12/26/2018

Proteome Profiler Human XL Cytokine Array Kit
By Scott Scarneo on 12/12/2018

THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/14/2018

Proteome Profiler Human XL Cytokine Array Kit
By Leslie Priddy on 04/01/2018