Proteome Profiler Human XL Cytokine Array Kit
Proteome Profiler Human XL Cytokine Array Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.
Key Benefits
- Detects 105 human cytokines simultaneously
- Requires no specialized equipment
- Compatible with LI-COR and chemiluminescence detection
Principle of the Array
The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
- Rectangular 4-Well Multi-dish
- 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Human XL Cytokine Array
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
Adiponectin/Acrp30 | IFN-gamma | CCL2/MCP-1 |
Angiogenin | IGFBP-2 | CCL7/MCP-3 |
Angiopoietin-1 | IGFBP-3 | M-CSF |
Angiopoietin-2 | IL-1 alpha/IL-1F1 | MIF |
Apolipoprotein A1 | IL-1 beta/IL-1F2 | CXCL9/MIG |
BAFF/BLyS/TNFSF13B | IL-1ra/IL-1F3 | CCL3/CCL4 MIP-1 alpha/beta |
BDNF | IL-2 | CCL20/MIP-3 alpha |
CD14 | IL-3 | CCL19/MIP-3 beta |
CD30 | IL-4 | MMP-9 |
CD31/PECAM-1 | IL-5 | Myeloperoxidase |
CD40 Ligand/TNFSF5 | IL-6 | Osteopontin (OPN) |
Chitinase 3-like | IL-8 | PDGF-AA |
Complement Component C5/C5a | IL-10 | PDGF-AB/BB |
Complement Factor D | IL-11 | Pentraxin 3/TSF-14 |
C-Reactive Protein/CRP | IL-12 p70 | CXCL4/PF4 |
Cripto-1 | IL-13 | RAGE |
Cystatin C | IL-15 | CCL5/RANTES |
Dkk-1 | IL-16 | RBP4 |
DPPIV/CD26 | IL-17A | Relaxin-2 |
EGF | IL-18 BPa | Resistin |
CXCL5/ENA-78 | IL-19 | CXCL12/SDF-1 alpha |
Endoglin/CD105 | IL-22 | Serpin E1/PAI-1 |
EMMPRIN | IL-23 | SHBG |
Fas Ligand | IL-24 | ST2/IL1 R4 |
FGF basic | IL-27 | CCL17/TARC |
KGF/FGF-7 | IL-31 | TFF3 |
FGF-19 | IL-32 alpha/beta/gamma | TfR |
Flt-3 Ligand | IL-33 | TGF-alpha |
G-CSF | IL-34 | Thrombospondin-1 |
GDF-15 | CXCL10/IP-10 | TIM-1 |
GM-CSF | CXCL11/I-TAC | TNF-alpha |
CXCL1/GRO alpha | Kallikrein 3/PSA | uPAR |
Growth Hormone (GH) | Leptin | VCAM-1 |
HGF | LIF | VEGF |
ICAM-1/CD54 | Lipocalin-2/NGAL | Vitamin D BP |
The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.
![]() View Larger Image |
Figure 1A. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours. |
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Figure 1B. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours. |
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Figure 1C. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours. |
Specifications
Product Datasheets
Background: Multiplex Cytokine Assay Kits
Citations for Proteome Profiler Human XL Cytokine Array Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Radiotherapy-Induced Changes in the Systemic Immune and Inflammation Parameters of Head and Neck Cancer Patients
Authors: K Balázs, E Kis, C Badie, EN Bogdándi, S Candéias, LC Garcia, I Dominczyk, B Frey, U Gaipl, Z Jurányi, ZS Kocsis, EA Rutten, G Sáfrány, P Widlak, K Lumniczky
Cancers (Basel), 2019;11(9):. 2019
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Abnormal inhibition of osteoclastogenesis by mesenchymal stem cells through the miR-4284/CXCL5 axis in ankylosing spondylitis
Authors: W Liu, P Wang, Z Xie, S Wang, M Ma, J Li, M Li, S Cen, S Tang, G Zheng, G Ye, X Wu, Y Wu, H Shen
Cell Death Dis, 2019;10(3):188. 2019
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Hypoxia Rapidly Induces the Expression of Cardiomyogenic Factors in Human Adipose-Derived Adherent Stromal Cells
Authors: JW Choi, H Moon, SE Jung, S Lim, S Lee, IK Kim, HB Lee, J Lee, BW Song, SW Kim, KC Hwang
J Clin Med, 2019;8(8):. 2019
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The Differences in the Characteristics of Insulin-producing Cells Using Human Adipose-tissue Derived Mesenchymal Stem Cells from Subcutaneous and Visceral Tissues
Authors: Y Wada, T Ikemoto, Y Morine, S Imura, Y Saito, S Yamada, M Shimada
Sci Rep, 2019;9(1):13204. 2019
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The Secretomes of Painful Versus Nonpainful Human Schwannomatosis Tumor Cells Differentially Influence Sensory Neuron Gene Expression and Sensitivity
Authors: KL Ostrow, KJ Donaldson, MJ Caterina, A Belzberg, A Hoke
Sci Rep, 2019;9(1):13098. 2019
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Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019;45(0):511-518. 2019
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The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method
Authors: D Kardos, M Simon, G Vácz, A Hinsenkamp, T Holczer, D Cseh, A Sárközi, K Szenthe, F Bánáti, S Szathmary, S Nehrer, O Kuten, M Masteling, Z Lacza, I Hornyák
Int J Mol Sci, 2019;20(3):. 2019
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Astrogliosis Releases Pro-Oncogenic Chitinase 3-Like 1 Causing MAPK Signaling in Glioblastoma
Authors: J Wurm, SP Behringer, VM Ravi, K Joseph, N Neidert, JP Maier, R Doria-Medi, M Follo, D Delev, D Pfeifer, J Beck, R Sankowski, O Schnell, DH Heiland
Cancers (Basel), 2019;11(10):. 2019
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Ectoine-Containing Inhalation Solution versus Saline Inhalation Solution in the Treatment of Acute Bronchitis and Acute Respiratory Infections: A Prospective, Controlled, Observational Study
Authors: BH Tran, VA Dao, A Bilstein, K Unfried, K Shah-Hosse, R Mösges
Biomed Res Int, 2019;2019(0):7945091. 2019
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Blockage of TGF-? Induced by Spherical Silica Nanoparticles Inhibits Epithelial-Mesenchymal Transition and Proliferation of Human Lung Epithelial Cells
Authors: Y Li, H Li, Y Duan, X Cai, D You, F Zhou, C Yang, X Tuo, Z Liu
Biomed Res Int, 2019;2019(0):8231267. 2019
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Obesity in acute alcoholic hepatitis increases morbidity and mortality
Authors: R Parker, SJ Kim, GY Im, J Nahas, B Dhesi, N Vergis, A Sinha, A Ghezzi, MR Rink, A McCune, GP Aithal, PN Newsome, CJ Weston, A Holt, B Gao
EBioMedicine, 2019;45(0):511-518. 2019
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Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature
Authors: LS Spitzhorn, M Megges, W Wruck, MS Rahman, J Otte, Ö Degistiric, R Meisel, RV Sorg, ROC Oreffo, J Adjaye
Stem Cell Res Ther, 2019;10(1):100. 2019
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p16INK4A-expressing mesenchymal stromal cells restore the senescence-clearance-regeneration sequence that is impaired in chronic muscle inflammation
Authors: TS Chikenji, Y Saito, N Konari, M Nakano, Y Mizue, M Otani, M Fujimiya
EBioMedicine, 2019;0(0):. 2019
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Genetic and pharmacological validation of TAK1 inhibition in macrophages as a therapeutic strategy to effectively inhibit TNF secretion
Authors: SA Scarneo, A Mansourati, LS Eibschutz, J Totzke, JR Roques, D Loiselle, D Carlson, P Hughes, TAJ Haystead
Sci Rep, 2018;8(1):17058. 2018
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Hypoxia-induced upregulation of angiogenic factors in immortalized human periodontal ligament fibroblasts
Authors: T Kifune, H Ito, M Ishiyama, S Iwasa, H Takei, T Hasegawa, M Asano, T Shirakawa
J Oral Sci, 2018;60(4):519-525. 2018
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Ca2+ Signaling and IL-8 Secretion in Human Testicular Peritubular Cells Involve the Cation Channel TRPV2
Authors: K Eubler, C Herrmann, A Tiefenbach, FM Köhn, JU Schwarzer, L Kunz, A Mayerhofer
Int J Mol Sci, 2018;19(9):. 2018
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Acid-electrolyzed functional water induces extracellular matrix metalloproteinase inducer, a possible novel alarmin, secretion from oral squamous cell carcinoma cell lines
Authors: M Kusunoki, E Sata, K Nishio, T Tanaka, T Nishida, N Sugano, S Sato, M Asano
Int J Med Sci, 2018;15(12):1365-1372. 2018
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USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and ?-TrCP and promotes cancer malignancy
Authors: YC Wang, YS Wu, CY Hung, SA Wang, MJ Young, TI Hsu, JJ Hung
Nat Commun, 2018;9(1):3996. 2018
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Neurofibromin knockdown in glioma cell lines is associated with changes in cytokine and chemokine secretion in vitro
Authors: MD Wood, J Mukherjee, RO Pieper
Sci Rep, 2018;8(1):5805. 2018
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ATP-mediated Events in Peritubular Cells Contribute to Sterile Testicular Inflammation
Authors: L Walenta, D Fleck, T Fröhlich, H von Eysmon, GJ Arnold, J Spehr, JU Schwarzer, FM Köhn, M Spehr, A Mayerhofer
Sci Rep, 2018;8(1):1431. 2018
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Identification and Complete Stereochemical Assignments of the New Resolvin Conjugates in Tissue Regeneration in Human Tissues that Stimulate Proresolving Phagocyte Functions and Tissue Regeneration
Authors: X de la Rosa, PC Norris, N Chiang, AR Rodriguez, BW Spur, CN Serhan
Am. J. Pathol., 2018;188(4):950-966. 2018
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A Transient Pseudosenescent Secretome Promotes Tumor Growth after Antiangiogenic Therapy Withdrawal
Authors: M Mastri, A Tracz, CR Lee, M Dolan, K Attwood, JG Christense, S Liu, JML Ebos
Cell Rep, 2018;25(13):3706-3720.e8. 2018
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CST3 and GDF15 ameliorate renal fibrosis by inhibiting fibroblast growth and activation
Authors: YI Kim, HW Shin, YS Chun, JW Park
Biochem. Biophys. Res. Commun., 2018;0(0):. 2018
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Withaferin A Associated Differential Regulation of Inflammatory Cytokines
Authors: S Dubey, H Yoon, MS Cohen, P Nagarkatti, M Nagarkatti, D Karan
Front Immunol, 2018;9(0):195. 2018
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Gle1 mediates stress granule-dependent survival during chemotoxic stress
Authors: L Glass, SR Wente
Adv Biol Regul, 2018;0(0):. 2018
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Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
Cell Death Dis, 2018;9(5):506. 2018
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Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
J. Cell. Mol. Med., 2018;0(0):. 2018
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Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
Cancer Med, 2018;0(0):. 2018
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A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
J Oral Sci, 2018;0(0):. 2018
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Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
Front Physiol, 2018;9(0):1025. 2018
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Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
Nat Commun, 2018;9(1):3267. 2018
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Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
Cell Rep, 2018;24(9):2300-2311. 2018
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Mesenchymal stem cells over-expressing cxcl12 enhance the radioresistance of the small intestine
Authors: P Chang, B Zhang, L Shao, W Song, W Shi, L Wang, T Xu, D Li, X Gao, Y Qu, L Dong, J Wang
Cell Death Dis, 2018;9(2):154. 2018
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Unique and shared inflammatory profiles of human brain endothelia and pericytes
Authors: LCD Smyth, J Rustenhove, TI Park, P Schweder, D Jansson, PA Heppner, SJ O'Carroll, EW Mee, RLM Faull, M Curtis, M Dragunow
J Neuroinflammation, 2018;15(1):138. 2018
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Efficient scalable production of therapeutic microvesicles derived from human mesenchymal stem cells
Authors: JM Cha, EK Shin, JH Sung, GJ Moon, EH Kim, YH Cho, HD Park, H Bae, J Kim, OY Bang
Sci Rep, 2018;8(1):1171. 2018
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Allele-specific long-distance regulation dictates IL-32 isoform switching and mediates susceptibility to HIV-1
Authors: RJ Palstra, E de Crignis, MD Röling, T van Staver, TW Kan, W van Ijcken, YM Mueller, PD Katsikis, T Mahmoudi
Sci Adv, 2018;4(2):e1701729. 2018
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IL-23 secreted by myeloid cells drives castration-resistant prostate cancer
Authors: A Calcinotto, C Spataro, E Zagato, D Di Mitri, V Gil, M Crespo, G De Bernard, M Losa, M Mirenda, E Pasquini, A Rinaldi, S Sumanasuri, MB Lambros, A Neeb, R Lucianò, CA Bravi, D Nava-Rodri, D Dolling, T Prayer-Gal, A Ferreira, A Briganti, A Esposito, S Barry, W Yuan, A Sharp, J de Bono, A Alimonti
Nature, 2018;0(0):. 2018
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Obesity Enhances the Conversion of Adipose-Derived Stromal/Stem Cells into Carcinoma-Associated Fibroblast Leading to Cancer Cell Proliferation and Progression to an Invasive Phenotype
Authors: AL Strong, DT Pei, CG Hurst, JM Gimble, ME Burow, BA Bunnell
Stem Cells Int, 2017;2017(0):9216502. 2017
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Histone Deacetylase 1 Depletion Activates Human Cardiac Mesenchymal Stromal Cell Proangiogenic Paracrine Signaling Through a Mechanism Requiring Enhanced Basic Fibroblast Growth Factor Synthesis and Secretion
Authors: JB Moore, J Zhao, AG Fischer, MCL Keith, D Hagan, M Wysoczynsk, R Bolli
J Am Heart Assoc, 2017;6(7):. 2017
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An autocrine/paracrine circuit of growth differentiation factor (GDF) 15 has a role for maintenance of breast cancer stem-like cells
Authors: A Sasahara, K Tominaga, T Nishimura, M Yano, E Kiyokawa, M Noguchi, M Noguchi, H Kanauchi, T Ogawa, H Minato, K Tada, Y Seto, A Tojo, N Gotoh
Oncotarget, 2017;8(15):24869-24881. 2017
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Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
J Am Heart Assoc, 2017;6(5):. 2017
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Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
Sci Rep, 2017;7(1):10608. 2017
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Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
J. Immunol., 2017;0(0):. 2017
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Crosstalk between stromal components and tumor cells of TNBC via secreted factors enhances tumor growth and metastasis
Authors: K Jin, NB Pandey, AS Popel
Oncotarget, 2017;0(0):. 2017
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Protective Effect of Let-7 miRNA Family in Regulating Inflammation in Diabetes-Associated Atherosclerosis
Authors: E Brennan, B Wang, A McClelland, M Mohan, M Marai, O Beuscart, S Derouiche, S Gray, R Pickering, C Tikellis, M de Gaetano, M Barry, O Belton, ST Ali-Shah, P Guiry, KA Jandeleit-, ME Cooper, C Godson, P Kantharidi
Diabetes, 2017;0(0):. 2017
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Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
Sci Rep, 2016;6(0):29588. 2016
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Pretreatment of IL-1? enhances proliferation and chondrogenic potential of synovium-derived mesenchymal stem cells
Authors: Etsuko Matsumura
Cytotherapy, 2016;0(0):. 2016
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Transcriptional upregulation of c-MET is associated with invasion and tumor budding in colorectal cancer
Oncotarget, 2016;0(0):. 2016
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Carcinoma-astrocyte gap junctions promote brain metastasis by cGAMP transfer
Nature, 2016;533(7604):493-8. 2016
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Identification of Pathways in Liver Repair Potentially Targeted by Secretory Proteins from Human Mesenchymal Stem Cells
Int J Mol Sci, 2016;17(7):. 2016
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Diesel Exhaust Particles Upregulate Interleukins IL-6 and IL-8 in Nasal Fibroblasts
Authors: Jin Ah Kim
PLoS ONE, 2016;11(6):e0157058. 2016
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Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
PLoS Pathog, 2015;11(12):e1005319. 2015
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VSIG-3 as a ligand of VISTA inhibits human T-cell function.
Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
Immunology, 0;156(1):74-85. 0
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THP-1 Cells stimulated with LPS+IFN gamma for 24 hours.