Proteome Profiler Human XL Cytokine Array Kit

Contains 4 membranes - each spotted in duplicate with 105 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY022B
Product Details
Citations (12)
FAQs
Reviews (2)

Proteome Profiler Human XL Cytokine Array Kit Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected human cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, plasma, human milk and saliva.

Key Benefits

  • Detects 105 human cytokines simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection
 

Principle of the Array

The Proteome Profiler Human XL Cytokine Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Human XL Cytokine Array nitrocellulose membranes spotted with 105 different antibodies to human cytokines
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Human XL Cytokine Array
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

 

Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
Adiponectin/Acrp30 IFN-gamma CCL2/MCP-1
Angiogenin IGFBP-2 CCL7/MCP-3
Angiopoietin-1 IGFBP-3 M-CSF
Angiopoietin-2 IL-1 alpha/IL-1F1 MIF
Apolipoprotein A1 IL-1 beta/IL-1F2 CXCL9/MIG
BAFF/BLyS/TNFSF13B IL-1ra/IL-1F3 CCL3/CCL4 MIP-1 alpha/beta
BDNF IL-2 CCL20/MIP-3 alpha
CD14 IL-3 CCL19/MIP-3 beta
CD30 IL-4 MMP-9
CD31/PECAM-1 IL-5 Myeloperoxidase
CD40 Ligand/TNFSF5 IL-6 Osteopontin (OPN)
Chitinase 3-like IL-8 PDGF-AA
Complement Component C5/C5a IL-10 PDGF-AB/BB
Complement Factor D IL-11 Pentraxin 3/TSF-14
C-Reactive Protein/CRP IL-12 p70 CXCL4/PF4
Cripto-1 IL-13 RAGE
Cystatin C IL-15 CCL5/RANTES
Dkk-1 IL-16 RBP4
DPPIV/CD26 IL-17A Relaxin-2
EGF IL-18 BPa Resistin
CXCL5/ENA-78 IL-19 CXCL12/SDF-1 alpha
Endoglin/CD105 IL-22 Serpin E1/PAI-1
EMMPRIN IL-23 SHBG
Fas Ligand IL-24 ST2/IL1 R4
FGF basic IL-27 CCL17/TARC
KGF/FGF-7 IL-31 TFF3
FGF-19 IL-32 alpha/beta/gamma TfR
Flt-3 Ligand IL-33 TGF-alpha
G-CSF IL-34 Thrombospondin-1
GDF-15 CXCL10/IP-10 TIM-1
GM-CSF CXCL11/I-TAC TNF-alpha
CXCL1/GRO alpha Kallikrein 3/PSA uPAR
Growth Hormone (GH) Leptin VCAM-1
HGF LIF VEGF
ICAM-1/CD54 Lipocalin-2/NGAL Vitamin D BP

 

Data Examples

The Human XL Cytokine Array detects multiple cytokines, chemokines, growth factors and other soluble proteins in cell culture supernates. Cells were untreated or treated as indicated below. 500 µL of cell culture supernate was run on each array. Data shown are from a 10 minute exposure to X-ray film. Profiles of mean spot pixel density were created using a transmission-mode scanner and image analysis software.

ARY022B Figure 1A
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Figure 1A. HUVEC human umbilical vein endothelial cells were untreated or treated with 100 µg/mL recombinant human TNF-alpha (Catalog #210-TA) for 24 hours.

ARY022B Figure 1B
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Figure 1B. SW480 human colorectal adenocarcinoma cells were untreated or treated with 100 ng/mL of LPS for 24 hours.

ARY022B Figure 1C
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Figure 1C. THP-1 human acute monocytic leukemia cells untreated or treated with 1 µg/mL of recombinant human IFN-gamma (Catalog #285-IF) for 16 hours and 1 µ/mL of LPS for 8 hours.

Product Datasheets

Preparation and Storage

Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Citations for Proteome Profiler Human XL Cytokine Array Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. Increased Dkk-1 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms
    Authors: C Mambet, L Necula, S Mihai, L Matei, C Bleotu, M Chivu-Econ, O Stanca, A Tatic, N Berbec, C Tanase, CC Diaconu
    J. Cell. Mol. Med., 2018;0(0):.  2018
  2. Epithelial cell-derived cytokines CST3 and GDF15 as potential therapeutics for pulmonary fibrosis
    Authors: YI Kim, HW Shin, YS Chun, CH Cho, J Koh, DH Chung, JW Park
    Cell Death Dis, 2018;9(5):506.  2018
  3. Circulating monocytes from prostate cancer patients promote invasion and motility of epithelial cells
    Authors: KA Cavassani, RJ Meza, DM Habiel, JF Chen, A Montes, M Tripathi, GA Martins, TR Crother, S You, CM Hogaboam, N Bhowmick, EM Posadas
    Cancer Med, 2018;0(0):.  2018
  4. A static magnetic field inhibits the expression of platelet-derived growth factor-AA in human oral squamous cell carcinoma
    Authors: M Obayashi-I, S Saito, D Omagari, M Asano, T Ishigami
    J Oral Sci, 2018;0(0):.  2018
  5. Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite
    Authors: N Li, C Wang, S Sun, C Zhang, D Lü, Q Chen, M Long
    Front Physiol, 2018;9(0):1025.  2018
  6. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.  2018
  7. Human Induced Pluripotent Stem Cell-Derived Microglia-Like Cells Harboring TREM2 Missense Mutations Show Specific Deficits in Phagocytosis
    Authors: P Garcia-Rei, A Phillips, TM Piers, C Villegas-L, M Butler, A Mallach, C Rodrigues, CE Arber, A Heslegrave, H Zetterberg, H Neumann, S Neame, H Houlden, J Hardy, JM Pocock
    Cell Rep, 2018;24(9):2300-2311.  2018
  8. Inflammatory Differences in Plaque Erosion and Rupture in Patients With ST-Segment Elevation Myocardial Infarction
    Authors: S Chandran, J Watkins, A Abdul-Aziz, M Shafat, PA Calvert, KM Bowles, MD Flather, SA Rushworth, AD Ryding
    J Am Heart Assoc, 2017;6(5):.  2017
  9. Extravillous Trophoblast and Endothelial Cell Crosstalk Mediates Leukocyte Infiltration to the Early Remodeling Decidual Spiral Arteriole Wall
    Authors: RH Choudhury, CE Dunk, SJ Lye, JD Aplin, LK Harris, RL Jones
    J. Immunol., 2017;0(0):.  2017
  10. Lack of Evidence for Vasoactive and Inflammatory Mediators in the Promotion of Macular Edema Associated with Epiretinal Membranes
    Authors: BP Applewhite, S Babapoor-F, D Poon, SJ Hassan, E Wellmann, HS Ying, GL Semenza, S Montaner, A Sodhi
    Sci Rep, 2017;7(1):10608.  2017
  11. Onionin A inhibits ovarian cancer progression by suppressing cancer cell proliferation and the protumour function of macrophages
    Sci Rep, 2016;6(0):29588.  2016
  12. Extracellular Histones Induce Chemokine Production in Whole Blood Ex Vivo and Leukocyte Recruitment In Vivo.
    Authors: Westman J, Papareddy P, Dahlgren M, Chakrakodi B, Norrby-Teglund A, Smeds E, Linder A, Morgelin M, Johansson-Lindbom B, Egesten A, Herwald H
    PLoS Pathog, 2015;11(12):e1005319.  2015

FAQs

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Reviews for Proteome Profiler Human XL Cytokine Array Kit

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Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 09/14/2018

Proteome Profiler Human XL Cytokine Array Kit
By Anonymous on 04/01/2018