PRR14 Antibody - BSA Free

Immunohistochemistry-Paraffin: PRR14 Antibody [NBP2-31812]

Immunohistochemistry-Paraffin: PRR14 Antibody [NBP2-31812] - Staining of human bronchus shows moderate nuclear, cytoplasmic and membranous positivity in respiratory epithelial cells.

Western Blot: PRR14 Antibody - BSA Free [NBP2-31812] -

PRR14 inhibits CHEK2.CHEK2 mRNA expression (a), CHEK2 protein expression (b) and p-CHEK2 (T68) protein expression (c) in PRR14 genetically unaltered and altered (amplified and mutated) breast cancer cases in TCGA database are statistically analyzed by two-tailed Student’s t-test. CHEK2 protein expression is detected by immunostaining in xenografts in nude mice from established MCF7 cell lines and MDA-MB-231 cell lines (d), as well as human breast cancer (e). CHEK2 transcription in human breast cancer is also detected by qRT-PCR (f). The data are quantified and two-tailed Student’s t-test is employed to determine the significance of the difference. Established MCF7 and MDA-MB-231 (g) PRR14-overexpressing and control cell lines are treated with various of genotoxic chemicals including Bleo, Eto, 5-FU, H2O2 and HU at indicated concentrations for indicated time. Key components of the ATM/CHEK2/P53 signaling pathway are detected by immunostaining. And CHEK2 protein expression (h) and mRNA expression (i) are detected by immunostaining and qRT-PCR, respectively. The data are analyzed by two-tailed Student’s t-test. Established MCF7 and MDA-MB-231 (j) PRR14-overexpressing and control cell lines are treated with Eto at indicated concentration for indicated time to induce p-CHEK2 (T68), which is detected by immunostaining and quantified and normalized by CHEK2 total protein (k). The data are analyzed by two-tailed Student’s t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32541902), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PRR14 Antibody - BSA Free [NBP2-31812] -

The upregulated expression of PRR14 is confirmed in human breast samples.PRR14 mRNA (a) and protein (b) expression in breast cancer and para-carcinoma breast tissue are detected by qRT-PCR and immunostaining, respectively. The protein level is quantified (c). Both the transcription and expression are normalized by ACTB and statistically analyzed by paired two-tailed Student’s t-test. Representative images of IHC staining for PRR14 with different staining intensity in a human breast cancer tissue microarray are presented (d). The data are presented as the percentage of samples with different PRR14 staining intensity (e, f). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/32541902), licensed under a CC-BY license. Not internally tested by Novus Biologicals.