PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

Novus Biologicals | Catalog # NBP2-02045

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H5
View all PSMA/FOLH1/NAALADase I Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human FOLH1(NP_004467) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-PSMA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunocytochemistry/ Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunocytochemistry/Immunofluorescence: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PSMA.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PSMA mouse monoclonal antibody.
Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Flow Cytometry: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PSMA antibody, and then analyzed by flow cytometry.
Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Western Blot: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PSMA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PSMA.
Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045]

Immunohistochemistry-Paraffin: PSMA/FOLH1/NAALADase I Antibody (OTI3H5) [NBP2-02045] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-PSMA mouse monoclonal antibody.

Applications for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Simple Western

1:50

Western Blot

1:500-1000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Prostate cell lysates, antibody dilution of 1:50

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.71 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSMA/FOLH1/NAALADase I

This gene encodes a type II transmembrane glycoprotein belonging to the M28 peptidase family. The protein acts as a glutamate carboxypeptidase on different alternative substrates, including the nutrient folate and the neuropeptide N-acetyl-l-aspartyl-l-glutamate and is expressed in a number of tissues such as prostate, central and peripheral nervous system and kidney. A mutation in this gene may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. Expression of this protein in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. In the prostate the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants encoding several different isoforms. [provided by RefSeq].

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human)

Gene Symbol

FOLH1

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for PSMA/FOLH1/NAALADase I Antibody (OTI3H5)

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  • Q: The spec sheet for this product does not indicate the type of antigen retrieval it will work well with. I have EDTA or citrate antigen retrieval. Will either one work just as well as the other?

    A:

    Either of your antigen retrieval solutions should work well for this antibody. Our lab used 0.01M Sodium Citrate Buffer, pH 6.0. Below was their protocol: Antigen Retrieval - Immerse slides into staining dish containing Antigen Retrieval Solution. Place covered staining dish into the rice cooker. Add 120 mL d H2O and press "cook". When "cook" is turned to "warm" (about 20-30 min), unplug the cooker and remove the staining dish to the bench top. Allow to cool down, without cover, for 20 min. Here is a link to our Antigen Retrieval Protocol.

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