PSMC3 Antibody (1G10B7) - BSA Free

Novus Biologicals | Catalog # NBP2-61802

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1G10B7

Format

BSA Free
View all PSMC3 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human PSMC3 (AA: 53-152) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSMC3 Antibody (1G10B7) - BSA Free

Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis using PSMC3 mAb against human PSMC3 (AA: 53-152) recombinant protein. (Expected MW is 37.2 kDa)
Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunocytochemistry/ Immunofluorescence: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunocytochemistry/Immunofluorescence: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis of MCF-7 cells using PSMC3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis of paraffin-embedded lung cancer tissues using PSMC3 mouse mAb with DAB staining.
Flow Cytometry: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Flow Cytometry: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Flow Cytometry: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis of Hela cells using PSMC3 mouse mAb (green) and negative control (red).
Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis using PSMC3 mouse mAb against COS7 (1), C6 (2), Hela (3), and A431 (4) cell lysate.;COS7,C6,Hela,A431
Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7)BSA Free [NBP2-61802]

Western Blot: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis using PSMC3 mAb against HEK293 (1) and PSMC3 (AA: 53-152)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

Immunohistochemistry-Paraffin: PSMC3 Antibody (1G10B7) [NBP2-61802] - Analysis of paraffin-embedded breast cancer tissues using PSMC3 mouse mAb with DAB staining.
ELISA: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

ELISA: PSMC3 Antibody (1G10B7) - BSA Free [NBP2-61802]

ELISA: PSMC3 Antibody (1G10B7) [NBP2-61802] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng).

Applications for PSMC3 Antibody (1G10B7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PSMC3

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.;

Alternate Names

human immunodeficiency virus tat transactivator binding protein-1, MGC8487, proteasome (prosome, macropain) 26S subunit, ATPase, 3,26S proteasome AAA-ATPase subunit RPT5, Proteasome 26S subunit ATPase 3, Proteasome subunit P50, TBP-126S protease regulatory subunit 6A, TBP1Tat-binding protein 1

Gene Symbol

PSMC3

Additional PSMC3 Products

Product Documents for PSMC3 Antibody (1G10B7) - BSA Free

Certificate of Analysis

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Product Specific Notices for PSMC3 Antibody (1G10B7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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