PSME4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-46861

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PSME4 Primary Antibodies »

Product Specifications

Immunogen

proteasome(prosome, macropain) activator subunit 4. (Uniprot# Q14997)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

140 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PSME4 Antibody - BSA Free

PSME4 Antibody - BSA Free

Western Blot: PSME4 Antibody - BSA Free [NBP3-46861] -

Western Blot: PSME4 Antibody - BSA Free [NBP3-46861] - HEK-293 cells were subjected to SDS PAGE followed by western blot with (PSME4 antibody) at dilution of 1:400
PSME4 Antibody - BSA Free

Immunoprecipitation: PSME4 Antibody - BSA Free [NBP3-46861] -

Immunoprecipitation: PSME4 Antibody - BSA Free [NBP3-46861] - IP Result of anti-PSME4 (IP:, 3ug; Detection: 1:500) with HEK-293 cells lysate 1760ug.
PSME4 Antibody - BSA Free

Immunohistochemistry: PSME4 Antibody - BSA Free [NBP3-46861] -

Immunohistochemistry: PSME4 Antibody - BSA Free [NBP3-46861] - Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using ( PSME4 antibody) at dilution of 1:200

Applications for PSME4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:100

Immunohistochemistry

1:50-1:500

Immunoprecipitation

1:500-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 50% Glycerol, pH (7.3)

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: PSME4

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. PA200 has been identified as a 200 kDa nuclear protein that activates the 26S proteasome complex. Studies have shown that, in human tissues, PA200 mRNA levels are highest in testis, but is also present in liver, spleen, brain, heart, kidney and lung. Evidence suggests that this protein is involved in DNA repair, possibly by recruiting the proteasome to double-strand DNA breaks.

Alternate Names

proteasome (prosome, macropain) activator subunit 4

Gene Symbol

PSME4

Additional PSME4 Products

Product Documents for PSME4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PSME4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for PSME4 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review PSME4 Antibody - BSA Free and earn rewards!

Have you used PSME4 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies