Human PTGER2 ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-00375

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma, cell supernatant, and other biological fluids (100 ul)

Sensitivity

37.5 pg/mL

Assay Range

62.5 - 4000 pg/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human PTGER2 in samples. No significant cross-reactivity or interference between Human PTGER2 and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) 4.78%

Inter-Assay Precision (Precision between assays) 4.83%

Recovery for Human PTGER2 ELISA Kit (Colorimetric)

Recovery

85 - 107%

Linearity

Human PTGER2 ELISA Kit (Colorimetric)

Scientific Data Images for Human PTGER2 ELISA Kit (Colorimetric)

Human PTGER2 ELISA Kit (Colorimetric)

ELISA: Human PTGER2 ELISA Kit (Colorimetric) [NBP3-00375] -

ELISA: Human PTGER2 ELISA Kit (Colorimetric) [NBP3-00375] - Standard Curve Reference

Kit Contents for Human PTGER2 ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100x)
  • Concentrated HRP Conjugate (100x)
  • Concentrated Wash Buffer (25x)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: PTGER2

Prostaglandin E2, a member of the autacoid family of lipid mediators, is a major renal cyclooxygenase product of arachidonic acid metabolism.Prostaglandin E2 binds to four G protein-coupled E-prostanoid receptors,designated EP1, EP2, EP3 and EP4. The expression and function of the prostaglandin E2 receptors have been highly characterized in kidney. EP1,which is predominantly expressed in the collecting duct, couples to Gq proteins to inhibit sodium absorption and increase in intracellular calcium, which act as second messengers. EP2 is coupled to Gs proteins, which stimulate adenylyl cyclase. EP2 has the lowest expression in kidney, but EP2 knockout mice exhibit salt-sensitive hypertension, which suggests a role for EP2 in salt excretion. EP3 is expressed in renal vessels, thick ascending limb and collecting duct. EP3 has at least 6 alternative splice variants that couple to Gi proteins to inhibit cAMP, which subsequently inhibit sodium and water transport. In uterus, EP3 induces the contraction of uterine smooth muscles.EP4 is expressed in glomerulus and collecting duct. It couples to Gs proteins,which stimulate adenylyl cyclase and regulate glomerular tone and renal renin release.

Long Name

Prostaglandin E2 Receptor 2, Subtype EP2

Alternate Names

EP2

Gene Symbol

PTGER2

Additional PTGER2 Products

Product Documents for Human PTGER2 ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human PTGER2 ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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