PTP1B/PTPN1 Antibody (226) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-89400

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 226 expressed in HEK293

Format

Azide and BSA Free
View all PTP1B Primary Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human PTP1B/PTPN1 (Accession#: NP_002818.1; Glu 2-Asn 321).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for PTP1B/PTPN1 Antibody (226) - Azide and BSA Free

Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400] - Lane A: PTPN1 konckout Hela Cell Lysate Lane B: Hela Whole Cell LysateLysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L /HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:50 kDa Observed band size:53 kDa
Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Western Blot: PTP1B/PTPN1 Antibody (226) [NBP2-89400] - Lane A: Hela whole cell lysate Lane B: MCF7 whole cell lysate Lane C: A549 whole cell lysate Lane D: Jurkat whole cell lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:50 kDa Observed band size:53 kDa
Immunoprecipitation: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Immunoprecipitation: PTP1B/PTPN1 Antibody (226) [NBP2-89400]

Immunoprecipitation: PTP1B/PTPN1 Antibody (226) [NBP2-89400] - Lane A:0.5 mg A549 Whole Cell Lysate 2 uL anti-PTPN1 rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PTPN1 rabbit monoclonal antibody,at 1:330 dilution Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 50 kDa Observed band size: 50 kDa

Applications for PTP1B/PTPN1 Antibody (226) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PTP1B

The non-transmembrane protein tyrosine phosphatase, PTP1B, comprises 435 amino acids, of which the C-terminal 114 residues have been implicated in controlling both localization and function of this enzyme. Increased phosphorylation of PTP1B is seen to accompany the transition from G2 to M phase of the cell cycle. Immunofluorescence experiments indicate that PTP-1B is localized predominantly in the endoplasmic reticulum (ER). Subcellular fractionation is consistent with this localization and establishes that PTP-1B is tightly associated with microsomal membranes, with its phosphatase domain oriented towards the cytoplasm. The finding of a tyrosine phosphatase on the ER suggests new possibilities for cellular events controlled by tyrosine phosphorylation (2). It has also been shown that PTP1B is a novel substrate for Akt and that phosphorylation of PTP1B by Akt at Ser(50) may negatively modulate its phosphatase activity creating a positive feedback mechanism for insulin signaling (3).

Long Name

Protein Tyrosine Phosphatase 1B

Alternate Names

PTPN1

Gene Symbol

PTPN1

Additional PTP1B Products

Product Documents for PTP1B/PTPN1 Antibody (226) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PTP1B/PTPN1 Antibody (226) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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