Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all PUM2 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 100 and 150 of human Pumilio 2 using the numbering given in entry NP_056132.1 (GeneID 23369).

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 25097251).

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

114 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for PUM2 Antibody - BSA Free

Western Blot: PUM2 Antibody [NB100-387]

Western Blot: PUM2 Antibody [NB100-387]

PUM2-Antibody-Western-Blot-NB100-387-img0003.jpg
Western Blot: PUM2 Antibody [NB100-387]

Western Blot: PUM2 Antibody [NB100-387]

Western Blot: PUM2 Antibody [NB100-387] - Detection of Human and Mouse Pumilio 2 by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-Pumilio 2 antibody NB100-387 used for WB at 0.2 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate.

Applications for PUM2 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

5-10 ug/mg lysate

Western Blot

1:10000-1:20000
Application Notes
By Western blot, this antibody recognizes bands at ~135-160 kDa, representing Pum2. A non-specific band at ~40 kDa may be seen in mouse lysates and just above 250 kDa in human lysates.

Reviewed Applications

Read 1 review rated 4 using NB100-387 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: PUM2

A component of conserved cellular machinery required for germ cell development, Pum2 is a human homolog of Pumilio, which helps maintain germ line stem cells in Drosophila and C. elegans. Pum2 forms a stable complex with DAZ through the same functional domain required for RNA binding and protein-protein interactions. Pum2 is expressed predominantly in human embryonic stem cells and germ cells and colocalizes with DAZ and DAZL in germ cells.

Long Name

Pumilio 2

Alternate Names

PUMH2, Pumilio-2, PUML2

Entrez Gene IDs

23369 (Human); 80913 (Mouse)

Gene Symbol

PUM2

UniProt

Q8TB72

Additional PUM2 Products

Product Documents for PUM2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for PUM2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PUM2 Antibody - BSA Free

Customer Reviews for PUM2 Antibody - BSA Free (1)

4 out of 5
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  • Name: Katherine Uyhazi
    Application: Western Blot
    Sample Tested: 293T whole cell lysate, Sample Amount: 20ug
    Species: Human
    Verified Customer | Posted 07/22/2010

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