Purine Nucleoside Phosphorylase/PNP Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-99935

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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Purine Nucleoside Phosphorylase/PNP Primary Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Purine Nucleoside Phosphorylase/PNP (Accession#: P00491; Met1-Ser289)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Purine Nucleoside Phosphorylase/PNP Antibody - Azide and BSA Free

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]

Western Blot: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa
Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]

Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935]

Immunoprecipitation: Purine Nucleoside Phosphorylase/PNP Antibody [NBP2-99935] - Purine Nucleoside Phosphorylase/PNP was immunoprecipitated using: Lane A: 0.5 mg Jurkat Whole Cell Lysate. Lane B: 0.5 mg k562 Whole Cell Lysate 2 ul anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Purine Nucleoside Phosphorylase/PNP rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 32 kDa. Observed band size: 32 kDa

Applications for Purine Nucleoside Phosphorylase/PNP Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Purine Nucleoside Phosphorylase/PNP

The enzyme purine nucleoside phosphorylase, together with adenosine deaminase (ADA) serves a key role in purine catabolism. Mutations in either enzyme result in a severe combined immunodeficiency (SCID).

Alternate Names

Inosine phosphorylase, PUNP

Gene Symbol

PNP

Additional Purine Nucleoside Phosphorylase/PNP Products

Product Documents for Purine Nucleoside Phosphorylase/PNP Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Purine Nucleoside Phosphorylase/PNP Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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