PYHIN1 Antibody - BSA Free

Western Blot: PYHIN1 Antibody [NBP1-79436]

Western Blot: PYHIN1 Antibody [NBP1-79436] - 721_B cell lysate, concentration 0.2-1 ug/ml.

Western Blot: PYHIN1 Antibody - BSA Free [NBP1-79436] -

Silencing NKD2 induces EMT in CAL‐27 cells with overexpressed or silenced IFIX. (A) qRT‐PCR analysis quantifying NKD2 expression in CAL‐27 cells with stable expression of shRNA molecules. NKD2 expression levels in CAL‐27 cells with various treatments, including control, overexpressed IFIX (OE‐IFIX) and silenced IFIX (shIFIX), are shown. (B) Western blot analysis of NKD2 and IFIX protein levels in CAL‐27 cells with different treatments: vector control, OE‐IFIX, NC + NC, sh‐NKD2 and OE‐IFIX + sh‐NKD2. (C) Quantification of IFIX protein levels from Western blot analysis. (D) Quantification of NKD2 protein levels from Western blot analysis. (E) Western blot analysis of EMT markers (E‐cadherin, N‐cadherin, Snail and vimentin) in response to IFIX silencing by siRNA molecules in CAL‐27 cells with different treatments. (F‐I) Quantification of E‐cadherin, N‐cadherin, vimentin and Snail protein levels from Western blot analysis. Vector: OE‐IFIX CAL‐27‐vector cells; OE‐IFIX: overexpressed IFIX‐CAL‐27 cells; sh‐NKD2: Sh‐NKD2‐CAL‐27 cells; NC + NC: OE‐IFIX‐CAL‐27‐vector + sh‐NKD2 CAL‐27‐vector cells; OE‐IFIX + sh‐NKD2: OE‐IFIX + sh‐NKD2‐CAL‐27 cells. Error bars indicate mean +/- SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39833105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: PYHIN1 Antibody - BSA Free [NBP1-79436] -

Effects of NKD2 and IFIX on tumour growth and EMT in vivo. (A) Tumour growth curves of SCC‐25 cells with stable expression of sh‐NKD2, OE‐IFIX, OE‐IFIX + sh‐NKD2 or NC + NC grafted into nude mice (n = 4). Tumour volume was measured over time. Error bars indicate mean +/- SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (B) Images of tumours excised from nude mice at the end of the experiment. (C) Immunohistochemistry (IHC) analysis of EMT markers (E‐cadherin, N‐cadherin, vimentin and Snail) and NKD2 expression in tumour tissues from NC + NC, sh‐NKD2, OE‐IFIX and OE‐IFIX + sh‐NKD2 groups. Images captured at 20× magnification. (D) Western blot analysis of IFIX and NKD2 protein levels in tumour tissues, with GAPDH as a loading control. (E) Quantification of tumour weights from excised tumours. Error bars indicate mean +/- SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (F) Quantification of relative protein levels of IFIX and NKD2 from Western blot analysis. Error bars indicate mean +/- SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39833105), licensed under a CC-BY license. Not internally tested by Novus Biologicals.