PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

Novus Biologicals | Catalog # H00002185-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1F9

Format

Azide and BSA Free
View all PYK2/FAK2 Primary Antibodies »

Product Specifications

Immunogen

PTK2B (AAH36651, 682 a.a. ~ 871 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VYQMEKDIAMEQERNARYRTPKILEPTAFQEPPPKPSRPKYRPPPQTNLLAPKLQFQVPEGLCASSPTLTSPMEYPSPVNSLHTPPLHRHNVFKRHSMREEDFIQPSSREEAQQLWEAEKVKMRQILDKQQKQMVEDYQWLRQEEKSLDPMVYMNDKSPLTPEKEVGYLEFTGPPQKPPRLGAQSIQPTA

Specificity

PTK2B - PTK2B protein tyrosine kinase 2 beta

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B expression in transfected 293T cell line by PTK2B monoclonal antibody (M01), clone 1F9.Lane 1: PTK2B transfected lysate(115.9 KDa).Lane 2: Non-transfected lysate.
Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Western Blot: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - PTK2B monoclonal antibody (M01), clone 1F9 Analysis of PTK2B expression in K-562.
Immunoprecipitation: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Immunoprecipitation: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

Immunoprecipitation: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Analysis of PTK2B transfected lysate using anti-PTK2B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTK2B MaxPab rabbit polyclonal antibody.
ELISA: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

ELISA: PYK2/FAK2 Antibody (1F9) [H00002185-M01]

ELISA: PYK2/FAK2 Antibody (1F9) [H00002185-M01] - Detection limit for recombinant GST tagged PTK2B is approximately 0.1ng/ml as a capture antibody.

Applications for PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: PYK2/FAK2

This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene.

Long Name

Proline-rich Tyrosine Kinase 2

Alternate Names

CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK

Entrez Gene IDs

2185 (Human)

Gene Symbol

PTK2B

OMIM

601212 (Human)

UniProt

AAH36651 (Human)

Additional PYK2/FAK2 Products

Product Documents for PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for PYK2/FAK2 Antibody (1F9) - Azide and BSA Free

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