PYK2/FAK2 [p Tyr402] Antibody (M269)

Novus Biologicals | Catalog # NBP3-23222

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M269
View all PYK2/FAK2 Primary Antibodies »

Product Specifications

Immunogen

Clone M269 was generated from a phosphopeptide containing amino acids surrounding tyrosine 402 in human PYK2/FAK2. This sequence is conserved in rat and mouse PYK2/FAK2, and has significant homology to the conserved site in FAK. [UniProt# Q14289]

Modification

p Tyr402

Specificity

The antibody detects a 116 kDa band corresponding to PYK2/FAK2 on SDS-PAGE immunoblots of human A431, Jurkat and K562 cells treated with pervanadate, but is not observed in control, untreated cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

116 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for PYK2/FAK2 [p Tyr402] Antibody (M269)

PYK2/FAK2 [p Tyr402] Antibody (M269)

Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] -

Western Blot: PYK2/FAK2 [p Tyr402] Antibody (M269) [NBP3-23222] - Western blot analysis of human Jurkat cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-PYK2/FAK2 (C-terminal region) antibody (lanes 1 & 2) or anti-PYK2/FAK2 (Tyr-402) (lanes 3 & 4).

Applications for PYK2/FAK2 [p Tyr402] Antibody (M269)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: PYK2/FAK2

Proline rich protein tyrosine kinase (Pyk2) is a focal adhesion kinase (FAK2) related protein kinase protein also known as cell adhesion kinase (CAK ), related adhesion focal tyrosine kinase (RAFTK), or calcium-dependent PTK (CADTK) (3.4). Highly expre ed in the nervous system, protein tyrosine kinase (Pyk2) is phosphorylated on the tyrosine residue by a variety of G-protein-coupled receptors & extracellular signals that increase the level of Ca+ intracellulary (1,2)

Long Name

Proline-rich Tyrosine Kinase 2

Alternate Names

CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK

Gene Symbol

PTK2B

Additional PYK2/FAK2 Products

Product Documents for PYK2/FAK2 [p Tyr402] Antibody (M269)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for PYK2/FAK2 [p Tyr402] Antibody (M269)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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