Rab11A Antibody (1F7-2A8) - Azide and BSA Free

Novus Biologicals | Catalog # H00008766-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Western Blot, ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F7-2A8

Format

Azide and BSA Free
View all Rab11A Primary Antibodies »

Product Specifications

Immunogen

RAB11A (AAH13348.1, 1 a.a. ~ 216 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI

Localization

Cytoplasmic

Marker

Recycling Endosome Marker

Specificity

RAB11A - RAB11A, member RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rab11A Antibody (1F7-2A8) - Azide and BSA Free

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8. Analysis of RAB11A expression in HepG2.
Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8. Analysis of RAB11A expression in PC-12.
Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01]

Western Blot: Rab11A Antibody (1F7-2A8) [H00008766-M01] - RAB11A monoclonal antibody (M01), clone 1F7-2A8 Analysis of RAB11A expression in K-562.
Sandwich ELISA: Rab11A Antibody (1F7-2A8) [H00008766-M01] - Detection limit for recombinant GST tagged RAB11A is approximately 1ng/ml as a capture antibody.

Applications for Rab11A Antibody (1F7-2A8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab11A

Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There are over 40 Rab proteins in mammals. Rab11a and Rab11b are known markers for protein trafficking, sorting, and recycling in the endosomal pathway. The Rab11 proteins are enriched in recycling endosomes and the trans-Golgi network, where they regulate membrane recycling back to the plasma membrane. Rab11a is ubiquitously expressed, while Rab11b is expressed mainly in the heart and brain.

Long Name

RAs Genes from Brain Protein 11A

Alternate Names

Rab-11, YL8

Entrez Gene IDs

8766 (Human)

Gene Symbol

RAB11A

OMIM

605570 (Human)

UniProt

AAH13348 (Human)

Additional Rab11A Products

Product Documents for Rab11A Antibody (1F7-2A8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rab11A Antibody (1F7-2A8) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epithelial Cell Polarity

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