Rab4 Antibody (1C10) - Azide and BSA Free

Novus Biologicals | Catalog # H00005867-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C10

Format

Azide and BSA Free
View all Rab4 Primary Antibodies »

Product Specifications

Immunogen

RAB4A (AAH04309, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSQTAMSETYDFLFKFLVIGNAGTGKSCLLHQFIEKKFKDDSNHTIGVEFGSKIINVGGKYVKLQIWDTAGQERFRSVTRSYYRGAAGALLVYDITSRETYNALTNWLTDARMLASQNIVIILCGNKKDLDADREVTFLEASRFAQENELMFLETSALTGENVEEAFVQCARKILNKIESGELDPERMGSGIQYGDAALRQLRSPRRAQAPNAQECGC

Localization

Peripheral membrane protein, generally associated with membranes. Cytoplasmic when phosphorylated by CDC2.

Marker

Early and Recycling Endosomes Marker

Specificity

RAB4A - RAB4A, member RAS oncogene family

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Rab4 Antibody (1C10) - Azide and BSA Free

Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - Analysis of RAB4A expression in transfected 293T cell line by RAB4A monoclonal antibody (M01), clone 1C10.Lane 1: RAB4A transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - Analysis of RAB4A over-expressed 293 cell line, cotransfected with RAB4A Validated Chimera RNAi ( Cat # H00005867-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB4A monoclonal antibody (M01), clone 1C10 (Cat # H00005867-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01]

Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - RAB4A monoclonal antibody (M01), clone 1C10 Analysis of RAB4A expression in Hela.
Sandwich ELISA: Rab4 Antibody (1C10) [H00005867-M01] - Detection limit for recombinant GST tagged RAB4A is approximately 0.1ng/ml as a capture antibody.

Applications for Rab4 Antibody (1C10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Entrez Gene IDs

5867 (Human)

Gene Symbol

RAB4A

OMIM

179511 (Human)

UniProt

AAH04309 (Human)

Additional Rab4 Products

Product Documents for Rab4 Antibody (1C10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Rab4 Antibody (1C10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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