Rab4 Antibody (4D0L8)

Novus Biologicals | Catalog # NBP3-33425

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 4D0L8
View all Rab4 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein of human Rab4.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rab4 Antibody (4D0L8)

Rab4 Antibody (4D0L8)

Western Blot: Rab4 Antibody (4D0L8) [NBP3-33425] -

Western Blot: Rab4 Antibody (4D0L8) [NBP3-33425] - Western blot analysis of various lysates using Rab4 Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 3min.

Applications for Rab4 Antibody (4D0L8)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Rab4

Rab4 is a small GTPase that regulates recycling of proteins, such as GPCRs, from the early endosomes to the cell surface. Overexpression of Rab4 causes a redistribution of receptors on plasma membrane versus endocytic compartments. Rab4 is also involved in the translocation of glucose transporter (Glu4) in adipocytes in response to insulin. Association of Rab4 with transferrin receptor containing early endosomes is mediated through the geranylgeranyl groups at its carboxyl terminus. Rab4 can be used as a marker for early endosomes (see Daro et al. 1996 and Zuk et al. 1999 for more information). Membrane association is also cell cycle dependent, as phosphorylation at its C-terminal cdc2 kinase consensus sequence in mitotic cells leads to dissociation of Rab4 into the cytosol.

Alternate Names

Oncogene RAB4, RAB4, member RAS oncogene family, RAB4A, member RAS oncogene family, RAB4HRES-1/RAB4, ras-related protein Rab-4A

Gene Symbol

RAB4A

Additional Rab4 Products

Product Documents for Rab4 Antibody (4D0L8)

Certificate of Analysis

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Product Specific Notices for Rab4 Antibody (4D0L8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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