Human SGK1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human SGK1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) SGK2 and rhSGK3 is observed.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SGK1
    Met1-Leu431
    Accession # O00141
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human SGK1 by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line non-transfected and transfected with human SGK1. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human SGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3200) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for SGK1 was detected at approximately 54 kDa (as indicated). For additional reference, recombinant human SGK1, SGK2, and SGK3 were included. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SGK1

Serum- and glucocorticoid-regulated protein kinase 1 (SGK1) is a member of the AGC family of serine/threonine kinases. In addition to serum and glucocorticoids, insulin, IGF-I, osmotic shock, and mineralocorticoids have been demonstrated to activate SGK1. Expressed at low levels, SGK1 appears to function as a regulator of epithelial ion transport. Sustained high levels of SGK1 activation have been implicated in hypertension and diabetic nephropathy.

  • Long Name:
    Serum/Glucocorticoid Regulated Kinase 1
  • Entrez Gene IDs:
    6446 (Human); 20393 (Mouse); 29517 (Rat)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.1; serine/threonine protein kinase SGK; serine/threonine-protein kinase Sgk1; serum/glucocorticoid regulated kinase 1; serum/glucocorticoid regulated kinase; Serum/glucocorticoid-regulated kinase 1; SGK; SGK1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Rabbit IgG Control

Ctrl AB-105-C 43  
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Normal Rabbit IgG Control

Ctrl, CyTOF-ready MAB1050  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rabbit IgG HRP-conjugated Antibody

WB HAF008 23  
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Donkey Anti-Rabbit IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL004 10
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Donkey Anti-Rabbit IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL005 2
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Rabbit IgG APC-conjugated Antibody

Flow F0111 1  
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Rabbit IgG PE-conjugated Antibody

Flow F0110 2  
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Rabbit IgG Fluorescein-conjugated Antibody

Flow F0112 5  
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Donkey Anti-Rabbit IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL006 4
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Goat Anti-Rabbit IgG Biotinylated Antibody

WB BAF008 4
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Rabbit IgG Antibody

WB AF008 1
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Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC003  
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Donkey Anti-Rabbit IgG (H+L) Antibody

D-301-C-ABS2
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