RAD18 Antibody

Novus Biologicals | Catalog # NB100-61063

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RAD18 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 445 and 495 of human E3 ubiquitin-protein ligase RAD18 using the numbering given in entry NP_064550.2 (GeneID 56852).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RAD18 Antibody

Western Blot: RAD18 Antibody [NB100-61063]

Western Blot: RAD18 Antibody [NB100-61063]

Western Blot: RAD18 Antibody [NB100-61063] - Detection of Human RAD18 on HeLa whole cell lysate using NB100-61063. RAD18 was also immunoprecipitated by rabbit anti-RAD18 antibody NB100-61062.

Applications for RAD18 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
KO Validation was reported in scientific literature (PMID: 27146073).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RAD18

The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Alternate Names

EC 6.3.2.-, hHR18, hRAD18, postreplication repair protein hRAD18p, Postreplication repair protein RAD18, RAD18 homolog (S. cerevisiae), RAD18, S. cerevisiae, homolog, RING finger protein 73, RNF73E3 ubiquitin-protein ligase RAD18

Entrez Gene IDs

56852 (Human)

Gene Symbol

RAD18

UniProt

Q9NS91 (Human)

Additional RAD18 Products

Product Documents for RAD18 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RAD18 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for RAD18 Antibody

There are currently no reviews for this product. Be the first to review RAD18 Antibody and earn rewards!

Have you used RAD18 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies