Rad50 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NB100-1487

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Rad50 Primary Antibodies »

Product Specifications

Immunogen

Human RAD50 zinc hook region (amino acids 628-787) expressed in E. coli. [UniProt# Q92878]

Localization

Upon DNA damage, Rad50 and Mre11 redistribute and are co-localized within the nucleus of damaged cells.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

153 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Rad50 Antibody - Azide and BSA Free

Western Blot: Rad50 Antibody [NB100-1487]

Western Blot: Rad50 Antibody [NB100-1487]

Western Blot: Rad50 Antibody [NB100-1487] - Detection of Rad50 using NB100-1487. Lane 1: 25 ng purified hMre11-hRad50 complex Lane 2: 25 ug crude HeLa extract.
Simple Western: Rad50 Antibody [NB100-1487]

Simple Western: Rad50 Antibody [NB100-1487]

Simple Western: Rad50 Antibody [NB100-1487] - Simple Western lane view shows a specific band for Rad50 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for Rad50 Antibody - Azide and BSA Free

Application
Recommended Usage

Immunoprecipitation

1-5ul

Simple Western

1:1000

Western Blot

1:1000-1:10000
Application Notes

This RAD50 antibody is useful for Immunoprecipitation and Western blot, where a band can be seen at approximately 153 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:1000, apparent MW was 149 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Rad50

RAD50 (DNA repair protein RAD50) is a component of MRN complex (Mre11-Rad50-Nbs1) that plays an important role in detection and signaling of DNA double strand breaks (DSBs) through acting as DSB sensor, co-activator of DSB-induced cell cycle checkpoint signaling, and as repair-effector in two competing DSB repair pathways: homologous recombination (HR) and non-homologous end-joining (NHEJ). MRN complex also associates with telomeres at the ends of linear chromosomes, where it contributes to their maintenance. RAD50 is required to bind DNA ends for holding them in close proximity where it facilitates searches for short or long regions of sequence homology in the recombining DNA templates, and also stimulate the activity of DNA ligases and/or restrict MRE11A's nuclease activity to prevent nucleolytic degradation. DSBs can be caused by ionizing radiation, certain chemotherapy drugs, metabolic ROS, as errors during replication, by programmed enzymatic activities during meiosis/V(D)J recombination etc., and if left unrepaired, DSBs can generate chromosomal translocations, aneuploidy and carcinogenesis. Defects in RAD50 have been linked to Nijmegen breakage syndrome-like disorder (NBSLD).

Alternate Names

DNA repair protein RAD50, EC 3.6, EC 3.6.1.15, EC 3.6.3, EC 3.6.3.27, hRAD50, NBSLD, RAD50 (S. cerevisiae) homolog, RAD50 homolog (S. cerevisiae), RAD502, RAD50-2

Entrez Gene IDs

10111 (Human)

Gene Symbol

RAD50

UniProt

Q92878

Additional Rad50 Products

Product Documents for Rad50 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Rad50 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rad50 Antibody - Azide and BSA Free

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Protocols

View specific protocols for Rad50 Antibody - Azide and BSA Free (NB100-1487):

Rad50 Antibody:
Western blot

1. Gel/Transfer set up according to manufacturer's instructions (NuPAGE, Invitrogen).
2. Block: 1X TBST +5% milk (TBST: 100mM Tris pH 7.5, 150mM NaCl, 0.1% Tween) for 1 hour at room temperature.
3. Wash 3 times (5 minutes) in 1X TBST.
4. Primary Antibody: Rad50, hook serum (NB100-1487) diluted 1:5,000 in 1X TBST and incubated for 1 hour at room temperature.
5. Wash 3 times (10 minutes) in 1X TBST.
6. Secondary Antibody: Anti-rabbit IgG diluted in 1X TBST and incubated for 1 hour at room temperature.
7. Wash 3 times (5 minutes) in 1X TBST.
8. Expose: Chemiluminescent HRP for 15-60 seconds.

NOTE: This is the protocol used to obtain the image on the datasheet.

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FAQs

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