RalA/RalB Antibody (AT41E2) - BSA Free

Novus Biologicals | Catalog # NBP2-88840

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # AT41E2

Format

BSA Free
View all RalA/RalB Primary Antibodies »

Product Specifications

Immunogen

Recombinant human RalA/RalB (1-203aa) purified from E. coli

Specificity

Detects both human RalA and RalB protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for RalA/RalB Antibody (AT41E2) - BSA Free

Western Blot: RalA/RalB Antibody (AT41E2)BSA Free [NBP2-88840]

Western Blot: RalA/RalB Antibody (AT41E2)BSA Free [NBP2-88840]

Western Blot: RalA/RalB Antibody (AT41E2) [NBP2-88840] - The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Recombinant human RalA proteinLane 2.: Recombinant human RalB protein
Flow Cytometry: RalA/RalB Antibody (AT41E2) - BSA Free [NBP2-88840]

Flow Cytometry: RalA/RalB Antibody (AT41E2) - BSA Free [NBP2-88840]

Flow Cytometry: RalA/RalB Antibody (AT41E2) [NBP2-88840] - Flow cytometry analysis of RalA/RalB in HeLa cells. The cell was stained at 2-5ug for 1x10^6cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (dark gray), cells without incubation with primary and secondary antibody was used as the negative control (light gray).
RalA/RalB Antibody (AT41E2) [NBP2-88840] - The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human RalA/RalB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: NIH3T3 cell lysateLane 4.: PC3 cell lysateLane 5.: U87MG cell lysate

Applications for RalA/RalB Antibody (AT41E2) - BSA Free

Application
Recommended Usage

Flow Cytometry

2-5ug

Western Blot

1:1000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RalA/RalB

The product of this gene belongs to the small GTPase superfamily, Ras family of proteins. GTP-binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors. This gene encodes a low molecular mass ras-like GTP-binding protein that shares about 50% similarity with other ras proteins.

Long Name

Ras-like Protein A/B

Alternate Names

MGC48949, RAL, Ras family small GTP binding protein RALA, RAS-like protein A, ras-related protein Ral-A, v-ral simian leukemia viral oncogene homolog A (ras related)

Gene Symbol

RALA

Additional RalA/RalB Products

Product Documents for RalA/RalB Antibody (AT41E2) - BSA Free

Certificate of Analysis

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Product Specific Notices for RalA/RalB Antibody (AT41E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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