Ran Antibody (8D1A6) - BSA Free

Novus Biologicals | Catalog # NBP2-61832

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8D1A6

Format

BSA Free
View all Ran Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human RAN (AA: 1-216) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Ran Antibody (8D1A6) - BSA Free

Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against HEK293 (1) and RAN (AA: 1-216)-hIgGFc transfected HEK293 (2) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of HepG2 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunohistochemistry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded stomach cancer tissues using RAN mouse mAb with DAB staining.
Flow Cytometry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Flow Cytometry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Flow Cytometry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green) and negative control (red).
Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mAb against human RAN (AA: 1-216) recombinant protein. (Expected MW is 50.4 kDa)
Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6)BSA Free [NBP2-61832]

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - Analysis using RAN mouse mAb against Hela (1), NIH/3T3 (2), A431 (3), C6 (4), Jurkat (5), Hela (6), COS7 (7), and Jurkat (8) cell lysate.
Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of GC-7901 cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/ Immunofluorescence: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunocytochemistry/Immunofluorescence: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of Hela cells using RAN mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunohistochemistry: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

Immunohistochemistry: Ran Antibody (8D1A6) [NBP2-61832] - Analysis of paraffin-embedded cervical cancer tissues using RAN mouse mAb with DAB staining.
ELISA: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

ELISA: Ran Antibody (8D1A6) - BSA Free [NBP2-61832]

ELISA: Ran Antibody (8D1A6) [NBP2-61832] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Ran Antibody (8D1A6) - BSA Free

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] -

Western Blot: Ran Antibody (8D1A6) [NBP2-61832] - RanGTPase detected with Mouse Monoclonal Ran Antibody (8D1A6) in HEK293T and LNCaP C42. Lanes are labeled as follows: 1. Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4. LNCaP C42 5. LNCaP C42. Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with infrared scanner. Image from verified customer review.

Applications for Ran Antibody (8D1A6) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

Reviewed Applications

Read 1 review rated 5 using NBP2-61832 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ran

RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease.;

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Gene Symbol

RAN

Additional Ran Products

Product Documents for Ran Antibody (8D1A6) - BSA Free

Certificate of Analysis

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Product Specific Notices for Ran Antibody (8D1A6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ran Antibody (8D1A6) - BSA Free

Customer Reviews for Ran Antibody (8D1A6) - BSA Free (1)

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  • Ran Antibody (8D1A6) - BSA Free
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysates from prostate cancer cell lines, HEK 293 cell line and HEK 293 cell lysate
    Species: Human
    Verified Customer | Posted 05/31/2023
    RanGTPase detected with Novus Antibody in HEK293T and LNCaP C42. Lanes are labeled as follows: 1- Recombinant protein 2. HEK239T 3. HEK293T constitutively expressing GFP-tagged construct 4.LNCaP C42 5.LNCaP C42
    Recombinant RAN protein is loaded with HEK293T and LNCaP C42 prostate cell line. Cell lysates run on a 4-15% mini gel. The ladder is Precision Plus Protein Standards Kaleidoscope. Proteins were detected with a secondary anti-mouse conjugated with Alexa Fluor 680 and detected with Licor infrared scanner.
    Ran Antibody (8D1A6) - BSA Free NBP2-61832

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Protocols

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