Ran Antibody (ARAN1) - BSA Free

Novus Biologicals | Catalog # NB600-1082

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine, Xenopus

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Microarray

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # ARAN1

Format

BSA Free
View all Ran Primary Antibodies »

Product Specifications

Immunogen

Denatured recombinant protein (Human). Uniprot accession no. P6826

Epitope

The antibody epitope is located at the C-terminus (amino acids 207-216), which is highly negatively charged.

Reactivity Notes

Cross-reacts with Xenopus laevis. Not yet tested in other species. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Nuclear

Specificity

Ran (ARAN1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Ran Antibody (ARAN1) - BSA Free

Western Blot: Ran Antibody (ARAN1)BSA Free [NB600-1082]

Western Blot: Ran Antibody (ARAN1)BSA Free [NB600-1082]

Western Blot: Ran Antibody (ARAN1) [NB600-1082] - Whole extract of Jurkat cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Ran Clone: ARAN1 and developed using Goat Anti-Mouse IgG-ALP and a NBT/BCIP substrate.

Applications for Ran Antibody (ARAN1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Immunoprecipitation

1:10-1:500

Western Blot

0.5 - 1.0 ug/ml
Application Notes
WB: Predicted molecular weight: 25 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ran

Alternate Names

Androgen receptor-associated protein 24, ARA24Gsp1, GTPase Ran, GTP-binding nuclear protein Ran, guanosine triphosphatase Ran, member RAS oncogene family, OK/SW-cl.81, RAN, member RAS oncogene family, RanGTPase, Ras-like protein TC4, Ras-related nuclear protein, TC4

Entrez Gene IDs

5901 (Human); 19384 (Mouse)

Gene Symbol

RAN

Additional Ran Products

Product Documents for Ran Antibody (ARAN1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ran Antibody (ARAN1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Ran Antibody (ARAN1) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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