Ran Antibody (JE33-28)

Western Blot: Ran Antibody (JE33-28) [NBP3-32892]

Western Blot: Ran Antibody (JE33-28) [NBP3-32892] - Western blot analysis of Ran on different lysates with Rabbit anti-Ran antibody (NBP3-32892) at 1/1,000 dilution.

Lane 1: A549 cell lysate (15 ug/Lane)
Lane 2: HeLa cell lysate (15 ug/Lane)
Lane 3: HepG2 cell lysate (15 ug/Lane)
Lane 4: LoVo cell lysate (15 ug/Lane)
Lane 5: Jurkat cell lysate (15 ug/Lane)
Lane 6: MDA-MB-231 cell lysate (15 ug/Lane)
Lane 7: MCF7 cell lysate (15 ug/Lane)
Lane 8: RAW264.7 cell lysate (15 ug/Lane)
Lane 9: NIH/3T3 cell lysate (15 ug/Lane)
Lane 10: Rat thymus tissue lysate (30 ug/Lane)

Predicted band size: 24 kDa
Observed band size: 24 kDa

Exposure time: 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32892) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Flow Cytometry: Ran Antibody (JE33-28) [NBP3-32892]

Flow Cytometry: Ran Antibody (JE33-28) [NBP3-32892] - Flow cytometric analysis of HeLa cells labeling Ran.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32892, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).