RanBP1 Antibody

Novus Biologicals | Catalog # NB100-1443

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all RanBP1 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-LSVKEETKEDAEEKQ corresponding to C-Terminus according to NP_002873.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for RanBP1 Antibody

Western Blot: RanBP1 Antibody [NB100-1443]

Western Blot: RanBP1 Antibody [NB100-1443]

Western Blot: RanBP1 Antibody [NB100-1443] - Staining of A431 (A) with antibody at 0.03 ug/mL, HeLa (B) with antibody at 0.5 ug/mL and MCF7 (C) cell lysate with antibody at 0.1 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: RanBP1 Antibody [NB100-1443]

Immunocytochemistry/ Immunofluorescence: RanBP1 Antibody [NB100-1443]

Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443]

Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443]

Immunohistochemistry-Paraffin: RanBP1 Antibody [NB100-1443] - Staining of paraffin embedded Human Kidney with antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Flow Cytometry: RanBP1 Antibody [NB100-1443]

Flow Cytometry: RanBP1 Antibody [NB100-1443]

Flow Cytometry: RanBP1 Antibody [NB100-1443] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western Blot: RanBP1 Antibody [NB100-1443]

Western Blot: RanBP1 Antibody [NB100-1443]

Western Blot: RanBP1 Antibody [NB100-1443] - Staining of Human Duodenum lysate with antibody at 0.3 ug/mL (35ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: RanBP1 Antibody [NB100-1443]

Immunocytochemistry/ Immunofluorescence: RanBP1 Antibody [NB100-1443]

Immunocytochemistry/Immunofluorescence: RanBP1 Antibody [NB100-1443] - Immunofluorescence analysis of paraformaldehyde fixed HEK293 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Applications for RanBP1 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

3 - 5 ug/mL

Immunohistochemistry-Paraffin

3 - 5 ug/mL

Peptide ELISA

Detection limit 1:16000

Western Blot

0.03 - 0.5 ug/mL

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RanBP1

Transport of macromolecules into and out of the nucleus occurs through nuclear pore complexes (NPC). This energy-dependent process involves nucleoporins at the NPC and associated transport proteins and GTP. Ran binding protein 1 (RanBP 1) belongs to a family of proteins that bind Ran GTPase which is essential for mRNA processing, nuclear transport, cell cycle control, mitotic spindle assembly, and postmitotic nuclear re-assembly and nuclear architecture maintenance.

Alternate Names

HTF9A, MGC88701, RAN binding protein 1, Ran-binding protein 1, RanBP1, ran-specific GTPase-activating protein

Entrez Gene IDs

5902 (Human)

Gene Symbol

RANBP1

UniProt

NP_002873. (Human)

Additional RanBP1 Products

Product Documents for RanBP1 Antibody

Certificate of Analysis

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Product Specific Notices for RanBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RanBP1 Antibody

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Protocols

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